Biomaterial Database

Simultaneous gas chromatographic analysis of lower fatty acids, phenols and indoles using a glass capillary column. 1977

Y Hoshika

The simultaneous gas chromatographic separation of a mixture of 14 lower fatty acis, 11 phenols and 7 indoles has been effected by using a glass capillary column. Complete separation of the mixture has been obtained, except for phenol and o-cresol and o-ethylphenol and 3,5-xylenol whose peaks overlapped, and 2- and 3-methylindoles which were poorly separated. The optimum conditions are as follows: stationary phase, FFAP or PEG-20M; column (20 m X 0.28 mm I.D.) temperature, held for 1 min at 145 degrees (FFAP) or 130 degrees (PEG-20M); column oven, heated at 4 degrees/min from 145 or 130 to 180 degrees (FFAP) or 165 degrees (PEG-20M), maintained at 180 or 165 degrees for 30 min; carrier gas (helium) flow-rate, 0.6 ml/min (FFAP), 1.3 ml/min (PEG-20M); flame ionization detector. The method has been applied to the analysis of lower fatty acids, phenols and indoles in Japanese cigarette smoke, where the peaks were identified by the disappearance method using an alkaline pre-column.

UI	MeSH Term	Description	Entries
D007211	Indoles	Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
D007564	Japan	A country in eastern Asia, island chain between the North Pacific Ocean and the Sea of Japan, east of the Korean Peninsula. The capital is Tokyo.	Bonin Islands
D010636	Phenols	Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
D010947	Plants, Toxic	Plants or plant parts which are harmful to man or other animals.	Plants, Poisonous,Plant, Poisonous,Plant, Toxic,Poisonous Plant,Poisonous Plants,Toxic Plant,Toxic Plants
D002849	Chromatography, Gas	Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.	Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D005227	Fatty Acids	Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)	Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D005898	Glass	Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.
D012906	Smoke	Visible gaseous suspension of carbon and other particulate matter emitted from burning substances.
D014026	Nicotiana	A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; the dried leaves of Nicotiana tabacum are used for SMOKING.	Tobacco Plant,Nicotiana tabacum,Plant, Tobacco,Plants, Tobacco,Tobacco Plants