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Histochemical study of the epithelia of nasal polyps by biotinylated lectins and neoglycoprotein. A comparison with the normal human respiratory epithelium. 1997

S Hassid, and I Salmon, and M Brugmans, and S Dawance, and R Kiss, and H J Gabius, and A Danguy
E.N.T. Department, Erasme University Hospital, Université Libre de Bruxelles, Belgium.

Formalin-fixed, paraffin-embedded sections from human nasal polyps and the normal respiratory epithelium were glycohistochemically investigated. Three biotinylated lectins were used: peanut (Arachis hypogaea) agglutinin (PNA) which, binds to terminal galactose (beta 1-3) N-acetylgalactosamine residues that can be unmasked by a neuraminidase digestion; wheat germ (Triticum vulgare) agglutinin (WGA), which binds to N-acetylglucosamine and N-acetylneuraminic acids; and gorse seed (Ulex europaeus) agglutinin (UEA-1), which binds to L-fucose. In addition, the presence of accessible galactose (beta 1-3) N-acetylgalactosamine (T-antigen) glycan receptors (endolectins) was also assessed. The avidin-biotin-peroxidase complex (ABC) and diaminobenzidine (DAB) were used as chromogen. The ciliated cells of the normal respiratory epithelium and those of the pseudostratified epithelium of nasal polyps possess similar glycohistochemical characteristics suggesting no major alterations on the level of lectin-reactive carbohydrate epitopes as indicators of cellular glycosylations. Notably, this parameter can respond sensitively to changes in cell differentiation or activation. The basal and mucus-secreting cells in the two epithelia display different reactivity patterns emphasizing the presence of dissimilar sugar residues. Similarly, the dysplasia reflecting squamous epithelium of nasal polyps shows a distinct staining behaviour, indicative for disparate glycoconjugate display. Thus, quantitative differences in the lectin-selective staining of various cell types are detectable. The expression of T-antigen-bearing neoglycoprotein binding is weak and similar in both the normal epithelium and the pseudostratified epithelium lining nasal polyps. Only the most superficial cells of the squamous epithelium disclose a moderate labelling with this probe. These results indicate that further studies in this field are warranted, employing neoglycoproteins and also endolectins from human tissues to correlate glycobiological properties of the epithelium of the conducting airways and its diseased forms with functional features.

UI MeSH Term Description Entries
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009298 Nasal Polyps Focal accumulations of EDEMA fluid in the NASAL MUCOSA accompanied by HYPERPLASIA of the associated submucosal connective tissue. Polyps may be NEOPLASMS, foci of INFLAMMATION, degenerative lesions, or malformations. Nasal Polyp,Polyp, Nasal,Polyps, Nasal
D011956 Receptors, Cell Surface Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands. Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D012137 Respiratory System The tubular and cavernous organs and structures, by means of which pulmonary ventilation and gas exchange between ambient air and the blood are brought about. Respiratory Tract,Respiratory Systems,Respiratory Tracts,System, Respiratory,Tract, Respiratory
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005260 Female Females
D005690 Galactose An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood. D-Galactose,Galactopyranose,Galactopyranoside,D Galactose
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006651 Histocytochemistry Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods. Cytochemistry

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