Biomaterial Database

Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus. 1997

D Paton, and G Ibata, and J Sands, and A McGoldrick

Virology Department, Central Veterinary Laboratory (Weybridge), Surrey, UK. dpaton.wood.cv1@gtnet.gov.uk

An RT-PCR method was developed that amplified genetic material from the 5' end of the S protein gene of both transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), but discriminated between the two by the size of the product generated. A number of restriction endonuclease enzymes were assessed for recognition of the amplicons so produced. The assay was shown to detect viral RNA from all of the 26 different TGEV and PRCV isolates examined, covering a period from 1946 to 1996. Detection of TGEV in clinical specimens was possible using a spin column method to extract RNA and sensitivity was compared to virus isolation and antigen detection ELISA. The method could provide a means of confirming positive results from immunological screening tests such as FAT and ELISA, reducing the need for virus isolation and convalescent serology.

UI	MeSH Term	Description	Entries
D007421	Intestine, Small	The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.	Small Intestine,Intestines, Small,Small Intestines
D008562	Membrane Glycoproteins	Glycoproteins found on the membrane or surface of cells.	Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D003937	Diagnosis, Differential	Determination of which one of two or more diseases or conditions a patient is suffering from by systematically comparing and contrasting results of diagnostic measures.	Diagnoses, Differential,Differential Diagnoses,Differential Diagnosis
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D005760	Transmissible gastroenteritis virus	A species of CORONAVIRUS causing a fatal disease to pigs under 3 weeks old.	Gastroenteritis Virus of Swine,Gastroenteritis Virus, Porcine Transmissible,Porcine Transmissible Gastroenteritis Virus,TGE Virus,Transmissible Gastroenteritis Virus, Swine,Swine Gastroenteritis Virus,Swine Gastroenteritis Viruses,TGE Viruses
D005761	Gastroenteritis, Transmissible, of Swine	A condition of chronic gastroenteritis in adult pigs and fatal gastroenteritis in piglets caused by a CORONAVIRUS.	Gastroenteritis, Transmissible, Porcine,Porcine Gastroenteritis, Transmissible,Swine Transmissible Gastroenteritis,Transmissible Gastroenteritis of Swine,Porcine Transmissible Gastroenteritis,Gastroenteritides, Porcine Transmissible,Gastroenteritides, Swine Transmissible,Gastroenteritides, Transmissible Porcine,Gastroenteritis, Porcine Transmissible,Gastroenteritis, Swine Transmissible,Gastroenteritis, Transmissible Porcine,Porcine Gastroenteritides, Transmissible,Porcine Transmissible Gastroenteritides,Swine Transmissible Gastroenteritides,Transmissible Gastroenteritides, Porcine,Transmissible Gastroenteritides, Swine,Transmissible Gastroenteritis, Porcine,Transmissible Gastroenteritis, Swine,Transmissible Porcine Gastroenteritides,Transmissible Porcine Gastroenteritis
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012367	RNA, Viral	Ribonucleic acid that makes up the genetic material of viruses.	Viral RNA
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity

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