Biomaterial Database

Differences between contractions of fast and slow muscles after nerve grafting. 1997

A J Carvalho, and P J Evans, and N H McKee, and S E Mackinnon

Department of Surgery, University of Toronto, Ontario, Canada.

The nature of reinnervation of fast- and slow-twitch skeletal muscles, as assessed by contractile characteristics, was determined in a rat sciatic-nerve graft model. The isometric contractile function of the fast-twitch plantaris and slow-twitch soleus muscles from hindlimbs of adult male Lewis rats (225 to 250 g) was assessed at 16 weeks after sciatic-nerve grafting. A 3-cm interposition sciatic-nerve graft was performed in the following groups: fresh syngeneic (n = 10), fresh allogeneic (n = 11), 3-week stored allogeneic (n = 9), and freeze-thawed allogeneic (n = 9). A control group consisted of 8 normal unoperated rats. Contractile properties were assessed by stimulating the muscles indirectly via the sciatic nerve. At 16 weeks, soleus and plantaris muscle masses were 40 and 52 percent of controls, while their respective absolute tetanic forces (N) were less than 65 and 45 percent of controls. Analysis of time-dependent contractile parameters showed that the soleus/plantaris ratios for time to peak tension (TTP) and maximum rate of force development (df/dt) were not significantly altered following grafting. However, the ratio for half relaxation time (1/2RT) was significantly reduced from 4.44 +/- 0.62 toward a value of 1 following grafting. In this study, the authors found that, when fast and slow muscles were reinnervated from a common nerve, maintenance of differences in rate of force development supported selective reinnervation, while loss of differences in time of force relaxation supported random reinnervation.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009046	Motor Neurons	Neurons which activate MUSCLE CELLS.	Neurons, Motor,Alpha Motorneurons,Motoneurons,Motor Neurons, Alpha,Neurons, Alpha Motor,Alpha Motor Neuron,Alpha Motor Neurons,Alpha Motorneuron,Motoneuron,Motor Neuron,Motor Neuron, Alpha,Motorneuron, Alpha,Motorneurons, Alpha,Neuron, Alpha Motor,Neuron, Motor
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009416	Nerve Regeneration	Renewal or physiological repair of damaged nerve tissue.	Nerve Tissue Regeneration,Nervous Tissue Regeneration,Neural Tissue Regeneration,Nerve Tissue Regenerations,Nervous Tissue Regenerations,Neural Tissue Regenerations,Regeneration, Nerve,Regeneration, Nerve Tissue,Regeneration, Nervous Tissue,Regeneration, Neural Tissue,Tissue Regeneration, Nerve,Tissue Regeneration, Nervous,Tissue Regeneration, Neural
D011917	Rats, Inbred Lew	An inbred strain of rat that is used in BIOMEDICAL RESEARCH.	Rats, Inbred Lewis,Rats, Lew,Inbred Lew Rat,Inbred Lew Rats,Inbred Lewis Rats,Lew Rat,Lew Rat, Inbred,Lew Rats,Lew Rats, Inbred,Lewis Rats, Inbred,Rat, Inbred Lew,Rat, Lew
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012584	Sciatic Nerve	A nerve which originates in the lumbar and sacral spinal cord (L4 to S3) and supplies motor and sensory innervation to the lower extremity. The sciatic nerve, which is the main continuation of the sacral plexus, is the largest nerve in the body. It has two major branches, the TIBIAL NERVE and the PERONEAL NERVE.	Nerve, Sciatic,Nerves, Sciatic,Sciatic Nerves
D016067	Nerve Transfer	Surgical reinnervation of a denervated peripheral target using a healthy donor nerve and/or its proximal stump. The direct connection is usually made to a healthy postlesional distal portion of a non-functioning nerve or implanted directly into denervated muscle or insensitive skin. Nerve sprouts will grow from the transferred nerve into the denervated elements and establish contact between them and the neurons that formerly controlled another area.	Nerve Crossover,Neurotization,Crossover, Nerve,Crossovers, Nerve,Nerve Crossovers,Nerve Transfers,Neurotizations,Transfer, Nerve,Transfers, Nerve
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D018656	Muscle Fibers, Fast-Twitch	Skeletal muscle fibers characterized by their expression of the Type II MYOSIN HEAVY CHAIN isoforms which have high ATPase activity and effect several other functional properties - shortening velocity, power output, rate of tension redevelopment. Several fast types have been identified.	Muscle Fibers, Intermediate,Muscle Fibers, Type II,Muscle Fibers, White,Fast-Twitch Muscle Fiber,Fast-Twitch Muscle Fibers,Fiber, Fast-Twitch Muscle,Fiber, Intermediate Muscle,Fiber, White Muscle,Fibers, Fast-Twitch Muscle,Fibers, Intermediate Muscle,Fibers, White Muscle,Intermediate Muscle Fiber,Intermediate Muscle Fibers,Muscle Fiber, Fast-Twitch,Muscle Fiber, Intermediate,Muscle Fiber, White,Muscle Fibers, Fast Twitch,White Muscle Fiber,White Muscle Fibers