Biomaterial Database

Comparison of nasopharyngeal and throat swabs for the detection of Chlamydia pneumoniae and Mycoplasma pneumoniae by polymerase chain reaction. 1997

J Gnarpe, and A Lundbäck, and H Gnarpe, and B Sundelöf

Department of Clinical Microbiology, Gävle Central Hospital, Sweden.

Nasopharyngeal and throat swabs taken from 66 patients presenting at the Department of Infectious Disease with symptoms of upper and lower respiratory tract infection were analysed by use of the polymerase chain reaction (PCR) for Chlamydia pneumoniae and Mycoplasma pneumoniae. A total of 18 patients (27%) were positive by PCR for C. pneumoniae. All 18 patients were positive from throat swabs, and three were also positive from nasopharyngeal specimens. The difference between the outcome of PCR using throat and nasopharyngeal swabs was statistically significant. A total of 7 patients (10.6%) were positive for M. pneumoniae and of these, 6 were positive from throat swabs and 2 were positive from nasopharyngeal swabs. This difference was not statistically significant, probably due to the low numbers of positive patient specimens.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008875	Middle Aged	An adult aged 45 - 64 years.	Middle Age
D009177	Mycoplasma pneumoniae	Short filamentous organism of the genus Mycoplasma, which binds firmly to the cells of the respiratory epithelium. It is one of the etiologic agents of non-viral primary atypical pneumonia in man.	Eaton Agent
D009305	Nasopharynx	The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.	Rhinopharynx,Choanae,Nasopharynges,Nasopharynxes,Rhinopharynges,Rhinopharynxes
D010614	Pharynx	A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).	Throat,Pharynxs,Throats
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016993	Chlamydophila pneumoniae	A species of CHLAMYDOPHILA that causes acute respiratory infection, especially atypical pneumonia, in humans, horses, and koalas.	Chlamydia pneumoniae

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