Culture of epithelial cells from the thymus of children and laboratory animals has been used for more than two decades to evaluate both the nature of these cells and their importance in the selection and maturation of functional T cells. Especially by the use of serum-free cultures and by establishment of cell lines from cultured thymic epithelial cells (TEC), it has been possible to obtain basic information on morphology of subpopulations of TEC, including surface determinants of importance for interactions with T-cell precursors, and on the repertoire of cytokines secreted by different types of TEC. The available information, obtained by co-culture of pre-T cells and TEC, on the effects of TEC on the fate of pre-T cells suggests that cultured TEC/TEC lines are able both to secrete needed cytokines for T-cell development, and to deliver signals needed for T-cell selection. In vivo results showing cross-talk between TEC and T cells indicate that more careful evaluation of interactions between well-defined subtypes of cultured TEC and co-cultured subpopulations of pre-T cells (as well as macrophages/dendritic cells) will be of importance in evaluation of the function of the thymus.