Biomaterial Database

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): an immunocytochemical study by light and electron microscopy. 1997

B Przybylska-Gornowicz, and B Lewczuk, and M Møller

Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Agriculture and Technology, Olsztyn, Poland.

The pineal gland is richly innervated by the neuropeptide Y (NPY)-positive nerves that mostly exibit immunoreactivity for the enzyme tyrosine hydroxylase, a marker of sympathetic nerve fibers. NPY is synthesized as a part of larger prepromolecule. The present study was undertaken to demonstrate that the posttranslational processing of preproNPY resulted in the presence of C-terminal flanking peptide of NPY (CPON) in nerve fibers of the pig pineal gland. An immunohistochemical anti-CPON technique was done over mounted sections of perfusion-paraformaldehyde-fixed material. An immunocytochemical preembedding technique was done to study the CPON-positive nerve terminals under electron microscopy. The pig pineal gland is densely innervated by CPON-immunoreactive nerve fibers. These nerve fibers follow from the pineal capsule into the connective tissue septa and farther into the pineal parenchyma, where the varicose branches terminate between the pinealocytes. The fiber density was the highest in the peripheral and ventral parts of the gland. At the ultrastructural level, the CPON-immunoreactive nerve terminals were found in the perivascular spaces and in the parenchyma. The terminals contained small vesicles (30-40 nm in diameter), some of which showed an eccentrically located dense core. In addition, large clear vesicles (80-100 nm in diameter) were present. Some of the CPON-immunoreactive nerve terminals were found in close apposition to the pinealocyte cell membrane, making a synapticlike contact with the pinealocytes. Our results show the presence of dense CPON-IR innervation in the pig pineal gland. The ultrastructural localization of CPON-IR nerve terminals shows that the peptide can be released to both perivascular and intercellular spaces. The functional role for this peptide in pig pineal gland is still an open question.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D009412	Nerve Fibers	Slender processes of NEURONS, including the AXONS and their glial envelopes (MYELIN SHEATH). Nerve fibers conduct nerve impulses to and from the CENTRAL NERVOUS SYSTEM.	Cerebellar Mossy Fibers,Mossy Fibers, Cerebellar,Cerebellar Mossy Fiber,Mossy Fiber, Cerebellar,Nerve Fiber
D009478	Neuropeptide Y	A 36-amino acid peptide present in many organs and in many sympathetic noradrenergic neurons. It has vasoconstrictor and natriuretic activity and regulates local blood flow, glandular secretion, and smooth muscle activity. The peptide also stimulates feeding and drinking behavior and influences secretion of pituitary hormones.	Neuropeptide Y-Like Immunoreactive Peptide,Neuropeptide Tyrosine,Neuropeptide Y Like Immunoreactive Peptide,Tyrosine, Neuropeptide
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D010870	Pineal Gland	A light-sensitive neuroendocrine organ attached to the roof of the THIRD VENTRICLE of the brain. The pineal gland secretes MELATONIN, other BIOGENIC AMINES and NEUROPEPTIDES.	Epiphysis Cerebri,Pineal Body,Corpus Pineale,Gland, Pineal,Pineal Bodies,Pineal Glands
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog