Calcium-binding characteristics and conformational changes in metastasin, a member of S-100 protein family. 1997

E A Dukhanina, and A S Dukhanin, and Lomonosov MYu, and E M Lukanidin, and G P Georgiev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. buh@genom-ii.eimb.rssi.ru

Investigation of Ca(2+)-binding characteristics of metastasin (Mts-1) by competition with Fluo-3 revealed two types of Ca(2+)-binding sites in Mts-1 with the geometric mean of their dissociation constant (Kd) value of 2.6 microM for the two EF-sites. The Hill coefficient (nH) is 0.98. A substantial increase in the affinity of Mts-1 for Ca2+ and strong cooperative character of binding (kd = 0.2 microM, nH = 1.91) is observed in the presence of the target protein p37 isolated from mouse adenocarcinoma cell lines CSML-100 and CSML-0. Two different hydrophobic sites of binding with the fluorescent probe 2-(p-toludino)naphthalene-6-sulfonate (TNS) per Mts-1 molecule have been determined. The exposure of the hydrophobic binding sites of the first type are shown to be Ca(2+)-dependent and the hydrophobic binding sites of the second type are exposed independently of Ca2+ concentration. A decrease in the number of Ca(2+)-dependent hydrophobic centers in the presence of p37 protein was detected by measurements of TNS fluorescence.

UI MeSH Term Description Entries
D009282 Naphthalenesulfonates A class of organic compounds that contains a naphthalene moiety linked to a sulfonic acid salt or ester.
D009418 S100 Proteins A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution. Antigen S 100,Nerve Tissue Protein S 100,S100 Protein,S-100 Protein,S100 Protein Family,Protein, S100,S 100 Protein
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D002135 Calcium-Binding Proteins Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS. Calcium Binding Protein,Calcium-Binding Protein,Calcium Binding Proteins,Binding Protein, Calcium,Binding Proteins, Calcium,Protein, Calcium Binding,Protein, Calcium-Binding
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D000071999 S100 Calcium-Binding Protein A4 An S100 protein characterized by four helix bundles that form N- and C-terminal EF HAND MOTIFS. It functions as a homodimer and interacts with both intracellular and extracellular signaling proteins. Aberrant S100A4 activity is associated with NEOPLASM METASTASIS; FIBROSIS; and RHEUMATOID ARTHRITIS. Calvasculin Protein,Fibroblast-Specific Protein 1,Metastasin Protein,Placental Calcium-Binding Protein,S100A4 Protein,Calcium-Binding Protein, Placental,Fibroblast Specific Protein 1,Placental Calcium Binding Protein,S100 Calcium Binding Protein A4
D000230 Adenocarcinoma A malignant epithelial tumor with a glandular organization. Adenocarcinoma, Basal Cell,Adenocarcinoma, Granular Cell,Adenocarcinoma, Oxyphilic,Adenocarcinoma, Tubular,Adenoma, Malignant,Carcinoma, Cribriform,Carcinoma, Granular Cell,Carcinoma, Tubular,Adenocarcinomas,Adenocarcinomas, Basal Cell,Adenocarcinomas, Granular Cell,Adenocarcinomas, Oxyphilic,Adenocarcinomas, Tubular,Adenomas, Malignant,Basal Cell Adenocarcinoma,Basal Cell Adenocarcinomas,Carcinomas, Cribriform,Carcinomas, Granular Cell,Carcinomas, Tubular,Cribriform Carcinoma,Cribriform Carcinomas,Granular Cell Adenocarcinoma,Granular Cell Adenocarcinomas,Granular Cell Carcinoma,Granular Cell Carcinomas,Malignant Adenoma,Malignant Adenomas,Oxyphilic Adenocarcinoma,Oxyphilic Adenocarcinomas,Tubular Adenocarcinoma,Tubular Adenocarcinomas,Tubular Carcinoma,Tubular Carcinomas
D000814 Aniline Compounds Compounds that include the aminobenzene structure. Phenylamine,Phenylamines,Anilines,Compounds, Aniline
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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