BIOMAT Database Logo BIOMAT Database Logo

Identification of a variant B-specific neutralizing epitope on glycoprotein H of human herpesvirus-6. 1997

K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Department of Microbiology, Osaka University Medical School, Osaka University, Japan.

We have identified the human herpesvirus-6 variant B (HHV-6B)-specific neutralizing epitope on glycoprotein H (gH) which is recognized by monoclonal antibody (MAb) OHV3, with complement-independent neutralizing activity. HHV-6 gHs from HHV-6A (strain U1102) and HHV-6B (strain HST) were expressed in a T7-vaccinia virus transient expression system. OHV3 reacted with HST gH, but not with U1102 gH, in an immunoprecipitation assay and an indirect immunofluorescence assay. In addition, OHV3 reacted with chimeric gHs, formed between U1102 gH and HST gH, containing amino acids 272 to 422 of HST gH. Sequence comparison between U1102 and HST showed seven amino acid differences in this region. Site-specific mutations were introduced into these positions and then reactivity against OHV3 was investigated. The arginine at position 389 of HST gH was shown to be a determinant of the HHV-6B-specific reactivity of OHV3.

UI MeSH Term Description Entries
D009500 Neutralization Tests The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50). Neutralization Test,Test, Neutralization,Tests, Neutralization
D011993 Recombinant Fusion Proteins Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. Fusion Proteins, Recombinant,Recombinant Chimeric Protein,Recombinant Fusion Protein,Recombinant Hybrid Protein,Chimeric Proteins, Recombinant,Hybrid Proteins, Recombinant,Recombinant Chimeric Proteins,Recombinant Hybrid Proteins,Chimeric Protein, Recombinant,Fusion Protein, Recombinant,Hybrid Protein, Recombinant,Protein, Recombinant Chimeric,Protein, Recombinant Fusion,Protein, Recombinant Hybrid,Proteins, Recombinant Chimeric,Proteins, Recombinant Fusion,Proteins, Recombinant Hybrid
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D000939 Epitopes Sites on an antigen that interact with specific antibodies. Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D001120 Arginine An essential amino acid that is physiologically active in the L-form. Arginine Hydrochloride,Arginine, L-Isomer,DL-Arginine Acetate, Monohydrate,L-Arginine,Arginine, L Isomer,DL Arginine Acetate, Monohydrate,Hydrochloride, Arginine,L Arginine,L-Isomer Arginine,Monohydrate DL-Arginine Acetate
D014759 Viral Envelope Proteins Integral membrane proteins that are incorporated into the VIRAL ENVELOPE. They are glycosylated during VIRAL ASSEMBLY. Envelope Proteins, Viral,Viral Envelope Glycoproteins,Viral Envelope Protein,Virus Envelope Protein,Virus Peplomer Proteins,Bovine Leukemia Virus Glycoprotein gp51,Hepatitis Virus (MHV) Glycoprotein E2,LaCrosse Virus Envelope Glycoprotein G1,Simian Sarcoma Virus Glycoprotein 70,Viral Envelope Glycoprotein gPr90 (Murine Leukemia Virus),Viral Envelope Glycoprotein gp55 (Friend Virus),Viral Envelope Proteins E1,Viral Envelope Proteins E2,Viral Envelope Proteins gp52,Viral Envelope Proteins gp70,Virus Envelope Proteins,Envelope Glycoproteins, Viral,Envelope Protein, Viral,Envelope Protein, Virus,Envelope Proteins, Virus,Glycoproteins, Viral Envelope,Peplomer Proteins, Virus,Protein, Viral Envelope,Protein, Virus Envelope,Proteins, Viral Envelope,Proteins, Virus Envelope,Proteins, Virus Peplomer
D015654 Herpesvirus 6, Human Members of the ROSEOLOVIRUS genus of the Betaherpesvirales subfamily isolated from patients with AIDS and other LYMPHOPROLIFERATIVE DISORDERS. It infects and replicates in fresh and established lines of hematopoietic cells and cells of neural origin. It also appears to alter the activity of NK CELLS. HHV-6; (HBLV) antibodies are elevated in patients with AIDS; SJOGREN'S SYNDROME; SARCOIDOSIS; CHRONIC FATIGUE SYNDROME, and certain malignancies. HHV-6A is the most common cause of EXANTHEMA SUBITUM and has been implicated in encephalitis. When HHV-6 integrates into the host genome it is referred to as ciHVH-6. When such VIRUS INTEGRATION occurs into the germline it is referred to as iciHHV-6. HBLV,Herpesvirus 6A, Human,Herpesvirus 6B, Human,Human B-Lymphotropic Virus,Chromosomally Integrated Human Herpesvirus 6,Chromosomally Integrated Human Herpesvirus 6A,Chromosomally Integrated Human Herpesvirus 6B,HHV-6,HHV-6A,HHV-6B,HHV6,HHV6A,HHV6B,Human Herpesvirus 6,Human betaherpesvirus 6,Human betaherpesvirus 6A,Human betaherpesvirus 6B,Inherited Chromosomally Integrated Human Herpesvirus 6,Inherited Chromosomally Integrated Human Herpesvirus 6A,Inherited Chromosomally Integrated Human Herpesvirus 6B,ciHHV-6,ciHHV-6A,ciHHV-6B,ciHHV6,ciHHV6A,ciHHV6B,iciHHV-6,iciHHV-6A,iciHHV-6B,iciHHV6,iciHHV6A,iciHHV6B,B-Lymphotropic Virus, Human,B-Lymphotropic Viruses, Human,Human B Lymphotropic Virus,Human B-Lymphotropic Viruses,Human Herpesvirus 6A,Human Herpesvirus 6B,Human betaherpesvirus 6s
D016297 Mutagenesis, Site-Directed Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion. Mutagenesis, Oligonucleotide-Directed,Mutagenesis, Site-Specific,Oligonucleotide-Directed Mutagenesis,Site-Directed Mutagenesis,Site-Specific Mutagenesis,Mutageneses, Oligonucleotide-Directed,Mutageneses, Site-Directed,Mutageneses, Site-Specific,Mutagenesis, Oligonucleotide Directed,Mutagenesis, Site Directed,Mutagenesis, Site Specific,Oligonucleotide Directed Mutagenesis,Oligonucleotide-Directed Mutageneses,Site Directed Mutagenesis,Site Specific Mutagenesis,Site-Directed Mutageneses,Site-Specific Mutageneses

Related Publications

K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Identification of a variant A-specific neutralizing epitope on glycoprotein B (gB) of human herpesvirus-6 (HHV-6).
August 1996, Virology,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
A Novel Strain-Specific Neutralizing Epitope on Glycoprotein H of Human Cytomegalovirus.
August 2021, Journal of virology,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex associates with human CD46.
April 2003, Journal of virology,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Complementation of the function of glycoprotein H of human herpesvirus 6 variant A by glycoprotein H of variant B in the virus life cycle.
August 2012, Journal of virology,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Identification of a neutralizing epitope on glycoprotein gp58 of human cytomegalovirus.
May 1989, Journal of virology,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
The Neutralizing Linear Epitope of Human Herpesvirus 6A Glycoprotein B Does Not Affect Virus Infectivity.
March 2018, Journal of virology,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Characterization of a discontinuous neutralizing epitope on glycoprotein B of human cytomegalovirus.
August 2013, Journal of virology,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Identification of a Neutralizing Epitope on TOSV Gn Glycoprotein.
August 2021, Vaccines,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Identification of a neutralizing epitope within antigenic domain 5 of glycoprotein B of human cytomegalovirus.
January 2015, Journal of virology,
K Takeda, and M Haque, and T Sunagawa, and T Okuno, and Y Isegawa, and K Yamanishi
Identification of human herpesvirus 6 variants A and B by amplimer hybridization with variant-specific oligonucleotides and amplification with variant-specific primers.
October 1994, Journal of clinical microbiology,
Copied contents to your clipboard!