BIOMAT Database Logo BIOMAT Database Logo

Molecular analysis of dengue virus attenuation after serial passage in primary dog kidney cells. 1997

B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889-5607, USA. PURI@MAIL2.NMRI.NNMC.NAVY.MIL

The complete nucleotide sequences of the genomes of dengue-1 virus virulent 45AZ5 PDK-O and attenuated vaccine candidate strain 45AZ5 PDK-27 have been determined and compared with the dengue-1 virus Western Pacific (West Pac) 74 parent strain from which 45AZ5 PDK-O was derived. Twenty-five (0.23%) nucleotide and 10 (0.29%) amino acid substitutions occurred between parent strain dengue-1 virus West Pac 74 and virulent strain 45AZ5 PDK-O, which was derived from the parent by serial passage in diploid foetal rhesus lung (FRhL-2) and mutagenized with 5-azacytidine. These substitutions were preserved in the 45AZ5 PDK-27 vaccine. 45AZ5 PDK-O and PDK-27 strains, which differ by 27 passages in primary dog kidney (PDK) cells, show 25 (0.23%) nucleotide and 11 (0.32%) amino acid divergences. These comparative studies suggest that the changes which occurred between the West Pac 74 and 45AZ5 PDK-O strains may alter the biological properties of the virus but may not be important for attenuation. Important nucleotide base changes responsible for attenuation accumulated between 45AZ5 PDK-O and 27.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003716 Dengue Virus A species of the genus FLAVIVIRUS which causes an acute febrile and sometimes hemorrhagic disease in man. Dengue is mosquito-borne and four serotypes are known. Breakbone Fever Virus,Breakbone Fever Viruses,Dengue Viruses,Fever Virus, Breakbone,Fever Viruses, Breakbone,Virus, Breakbone Fever,Virus, Dengue,Viruses, Breakbone Fever,Viruses, Dengue
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D012692 Serial Passage Inoculation of a series of animals or in vitro tissue with an infectious bacterium or virus, as in VIRULENCE studies and the development of vaccines. Passage, Serial,Passages, Serial,Serial Passages
D014613 Vaccines, Attenuated Live vaccines prepared from microorganisms which have undergone physical adaptation (e.g., by radiation or temperature conditioning) or serial passage in laboratory animal hosts or infected tissue/cell cultures, in order to produce avirulent mutant strains capable of inducing protective immunity. Attenuated Vaccine,Vaccines, Live, Attenuated,Attenuated Vaccines,Vaccine, Attenuated

Related Publications

B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Study of biologic attributes of Cuban dengue 2 virus after serial passage in primary dog kidney cells.
January 2001, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. II. Attributes of virus cloned at different dog kidney passage levels.
July 1984, The American journal of tropical medicine and hygiene,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Attenuation of rubella virus by serial passage in primary rabbit kidney cells. 3. Clinical trials in infants.
January 1968, Pediatric research,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Biologic properties of dengue viruses following serial passage in primary dog kidney cells: studies at the University of Hawaii.
December 2003, The American journal of tropical medicine and hygiene,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Increasing attenuation of measles virus strain Sugiyama during serial passage in calf kidney cells.
August 1970, The Japanese journal of experimental medicine,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. I. Attributes of uncloned virus at different passage levels.
July 1984, The American journal of tropical medicine and hygiene,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Attenuation of rubella virus by serial passage in primary rabbit kidney cell cultures. II. Experiments in animals.
January 1967, Archiv fur die gesamte Virusforschung,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Modification of dengue virus strains by passage in primary dog kidney cells: preparation of candidate vaccines and immunization of monkeys.
December 2003, The American journal of tropical medicine and hygiene,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. III. Reversion to virulence by passage of cloned virus in fetal rhesus lung cells.
July 1984, The American journal of tropical medicine and hygiene,
B Puri, and W M Nelson, and E A Henchal, and C H Hoke, and K H Eckels, and D R Dubois, and K R Porter, and C G Hayes
Attenuation of hog-cholera virus by serial passage in rabbits.
December 1947, Journal of the American Veterinary Medical Association,
Copied contents to your clipboard!