BACKGROUND The interpretation of spirometric results requires reference values. Up to the moment, the reference values used in Chile are those of Knudson et al, obtained in a white North American population studied in Tucson, USA. OBJECTIVE To obtain reference spirometric values from a Chilean population. METHODS Maximal expiratory flow-volume curves were obtained from a randomly selected population of 850 healthy non-smoking subjects living at sea level, in cities without atmospheric pollution. Equipment and techniques were used according to the recommendations of the American Thoracic Society. RESULTS Linear regression equations were obtained, using age and height as independent variables and spirometric values as dependent variables, for both sexes and ages from 6 to 70 years old. Our values for forced vital capacity and forced expiratory volume were 9 to 18% and 10 to 15% higher than those reported by Knudson et al. CONCLUSIONS Chilean spirometric reference values differ from those used abroad, emphasizing the need to use local standards to interpret spirometric results.