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We describe a capillary electrophoresis procedure, using uridine as an internal standard, for the analysis of urinary pseudouridine following solid-phase extraction. This method retains the advantages of existing chromatographic techniques but has superior resolving power and is technically less demanding. The standard curves were linear and reproducible with a detection limit of 60 fmol; chromatographic analysis was complete in under 10 min. Injection variability was < 5% and multiple independent analyses of the same urine sample for pseudouridine concentration gave coefficients of variation of < 10%. The mean (SD) urinary pseudouridine level in 18 healthy subjects was 16.1 (2.1) nmol/mumol creatinine. For a limited group of subjects where samples were taken more frequently, intra-individual variation averaged 27.5% reflecting variable excretion.
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
January 1999,
Methods in molecular medicine,
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
February 1997,
Journal of chromatography. A,
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
June 2006,
Electrophoresis,
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
August 2003,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
August 1988,
Journal of chromatographic science,
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
January 2009,
Annual review of analytical chemistry (Palo Alto, Calif.),
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
March 1997,
Se pu = Chinese journal of chromatography,
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
August 1988,
Journal of chromatography,
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
February 1997,
Electrophoresis,
M D Evans, and
D Perrett, and
J Lunec, and
K E Herbert
May 1993,
Analytical biochemistry,
