BIOMAT Database Logo BIOMAT Database Logo

Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination In vivo. 1997

H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
Department of Medical Physics and Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York 10021, USA.

Ku is a complex of two proteins, Ku70 and Ku80, and functions as a heterodimer to bind DNA double-strand breaks (DSB) and activate DNA-dependent protein kinase. The role of the Ku70 subunit in DNA DSB repair, hypersensitivity to ionizing radiation, and V(D)J recombination was examined in mice that lack Ku70 (Ku70(-/-)). Like Ku80(-/-) mice, Ku70(-/-) mice showed a profound deficiency in DNA DSB repair and were proportional dwarfs. Surprisingly, in contrast to Ku80(-/-) mice in which both T and B lymphocyte development were arrested at an early stage, lack of Ku70 was compatible with T cell receptor gene recombination and the development of mature CD4+CD8- and CD4-CD8+ T cells. Our data shows, for the first time, that Ku70 plays an essential role in DNA DSB repair, but is not required for TCR V(D)J recombination. These results suggest that distinct but overlapping repair pathways may mediate DNA DSB repair and V(D)J recombination.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009687 Nuclear Proteins Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. Nucleolar Protein,Nucleolar Proteins,Nuclear Protein,Protein, Nuclear,Protein, Nucleolar,Proteins, Nuclear,Proteins, Nucleolar
D011948 Receptors, Antigen, T-Cell Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (CD3 COMPLEX). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains. Antigen Receptors, T-Cell,T-Cell Receptors,Receptors, T-Cell Antigen,T-Cell Antigen Receptor,T-Cell Receptor,Antigen Receptor, T-Cell,Antigen Receptors, T Cell,Receptor, T-Cell,Receptor, T-Cell Antigen,Receptors, T Cell Antigen,Receptors, T-Cell,T Cell Antigen Receptor,T Cell Receptor,T Cell Receptors,T-Cell Antigen Receptors
D011995 Recombination, Genetic Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses. Genetic Recombination,Recombination,Genetic Recombinations,Recombinations,Recombinations, Genetic
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004260 DNA Repair The removal of DNA LESIONS and/or restoration of intact DNA strands without BASE PAIR MISMATCHES, intrastrand or interstrand crosslinks, or discontinuities in the DNA sugar-phosphate backbones. DNA Damage Response
D004265 DNA Helicases Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition, DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands. ATP-Dependent DNA Helicase,DNA Helicase,DNA Unwinding Protein,DNA Unwinding Proteins,ATP-Dependent DNA Helicases,DNA Helicase A,DNA Helicase E,DNA Helicase II,DNA Helicase III,ATP Dependent DNA Helicase,ATP Dependent DNA Helicases,DNA Helicase, ATP-Dependent,DNA Helicases, ATP-Dependent,Helicase, ATP-Dependent DNA,Helicase, DNA,Helicases, ATP-Dependent DNA,Helicases, DNA,Protein, DNA Unwinding,Unwinding Protein, DNA,Unwinding Proteins, DNA
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D000072200 Ku Autoantigen An ATP-dependent DNA HELICASE that preferentially binds SINGLE-STRANDED DNA. It is a heterodimer consisting of an 80 kDa subunit (XRCC5) and 70 kDa subunit (XRCC6) that functions with DNA LIGASE IV in the repair of DOUBLE-STRANDED DNA BREAKS and V(D)J RECOMBINATION. G22P1 Antigen,Ku Antigen,Ku Autoantigen, 70 kDa,Ku Autoantigen, 80 kDa,Ku Heterodimer,Ku Protein,Ku70 Antigen,Ku80 Antigen,X-ray Repair Cross-Complementing Protein 5,X-ray Repair Cross-Complementing Protein 6,XRCC5 Protein,XRCC6 Protein,Antigen, G22P1,Antigen, Ku,Antigen, Ku70,Antigen, Ku80,Autoantigen, Ku,Heterodimer, Ku,X ray Repair Cross Complementing Protein 5,X ray Repair Cross Complementing Protein 6

Related Publications

H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA.
July 2003, Nucleic acids research,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
Replication factor A is required in vivo for DNA replication, repair, and recombination.
December 1994, Molecular and cellular biology,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
Ku80 plays a role in non-homologous recombination but is not required for T-DNA integration in Arabidopsis.
September 2003, The Plant journal : for cell and molecular biology,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
Proliferating cell nuclear antigen is required for DNA excision repair.
April 1992, Cell,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
T-cell gamma gene is allelically but not isotypically excluded and is not required in known functional T-cell subsets.
November 1987, Proceedings of the National Academy of Sciences of the United States of America,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
JNK is required for effector T-cell function but not for T-cell activation.
May 2000, Nature,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
The T-cell receptor beta variable gene promoter is required for efficient V beta rearrangement but not allelic exclusion.
August 2004, Molecular and cellular biology,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
T Cell Receptor Is Required for Differentiation, but Not Maintenance, of Intestinal CD4+ Intraepithelial Lymphocytes.
November 2020, Immunity,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
An essential gene, ESR1, is required for mitotic cell growth, DNA repair and meiotic recombination in Saccharomyces cerevisiae.
August 1994, Nucleic acids research,
H Ouyang, and A Nussenzweig, and A Kurimasa, and V C Soares, and X Li, and C Cordon-Cardo, and W h Li, and N Cheong, and M Nussenzweig, and G Iliakis, and D J Chen, and G C Li
Autophagy is critically required for DNA repair by homologous recombination.
January 2016, Molecular & cellular oncology,
Copied contents to your clipboard!