-transparent.png){kind=logo}
A general high-performance gel-permeation chromatography (HPGPC) method was developed to determine protein in human serum with improved sensitivity and speed. The optimum UV wavelength for protein detection was found to be 210 nm, by comparing the protein values obtained by varying the UV wavelength of the HPLC detection system with the protein values obtained from spectrophotometric protein assays, i.e., the bicinchoninic acid (BCA) method and the biuret method. The analysis time was less than 1 min. Since this HPGPC serum protein assay method is simple and rapid, it is expected to be particularly well adapted for use in clinical laboratories.
Related Publications
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
June 1982,
Journal of chromatography,
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
July 1993,
Journal of chromatography,
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
September 1983,
Analytical biochemistry,
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
November 1989,
Journal of chromatography,
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
December 1980,
Journal of chromatography,
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
May 1988,
Journal of chromatography,
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
December 1980,
Journal of chromatography,
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
February 1981,
Analytical biochemistry,
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
January 1984,
Methods in molecular biology (Clifton, N.J.),
K Hayakawa, and
M Masuko, and
M Mineta, and
K Yoshikawa, and
K Yamauchi, and
M Hirano, and
N Katsumata, and
T Tanaka
March 2011,
Se pu = Chinese journal of chromatography,
