-transparent.png){kind=logo}
To evaluate the role of mycobacterial infection in the pathogenesis of sarcoidosis, several groups have attempted to identify mycobacterial DNA in clinical samples from these patients by polymerase chain reaction (PCR), but widely divergent results have been obtained. It has been suggested that differences in the sensitivity of the procedures used may explain these discrepant results. To test this possibility, the presence of mycobacterial DNA was sought in biopsies from patients with sarcoidosis using sequence capture-PCR, a procedure that is 100-fold more sensitive in detecting mycobacterial DNA in paucibacillary samples than standard PCR protocols. Using this approach, DNA corresponding to two different sequences specific for organisms of the Mycobacterium tuberculosis complex (the 1S6110 insertion element and the DR region) could not be detected in any of the 15 biopsies from patients with sarcoidosis, whereas a high proportion of positive results was obtained for tissue biopsies and other clinical samples from patients with active tuberculosis, including samples that were smear-negative/culture-positive and smear-negative/culture-negative. These results support prior studies suggesting that M. tuberculosis does not play a pathogenic role in sarcoidosis in most patients.
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
May 1996,
Clinical and experimental dermatology,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
March 2001,
Diagnostic molecular pathology : the American journal of surgical pathology, part B,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
January 2005,
Archives of dermatology,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
June 2005,
Veterinary microbiology,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
December 2014,
International journal of mycobacteriology,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
June 1978,
Canadian journal of microbiology,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
January 1992,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
July 1999,
Laboratory investigation; a journal of technical methods and pathology,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
June 1988,
Molecular and cellular probes,
M Vokurka, and
D Lecossier, and
R M du Bois, and
B Wallaert, and
M Kambouchner, and
A Tazi, and
A J Hance
August 1965,
The American review of respiratory disease,
