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We have developed a liquid chromatography-isotope dilution mass spectrometry procedure to quantify total cholesterol in serum. A particle-beam interface was used for coupling the liquid chromatograph and the mass spectrometer. After electron impact ionization the ions m/z = 386 and m/z = 389 were used for selective ion monitoring of cholesterol and the internal standard [25,26,27-(13)C]cholesterol. The sample preparation steps required for serum materials are alkaline hydrolysis and an extraction of the cholesterol into the cyclohexane phase. Imprecision for the determination of cholesterol in control materials is typically <1.0%. The deviation from the certified reference values was <0.75% for all control materials tested. A method comparison of the results obtained by this method with those obtained by gas chromatography-isotope dilution mass spectrometry for n = 28 pooled human sera derived from samples analyzed in our routine laboratory did not show differences >2.5%.
R Kock, and
B Delvoux, and
H Greiling
April 2011,
Clinical chemistry and laboratory medicine,
R Kock, and
B Delvoux, and
H Greiling
October 1988,
Analytical chemistry,
R Kock, and
B Delvoux, and
H Greiling
April 1993,
Biological mass spectrometry,
R Kock, and
B Delvoux, and
H Greiling
January 2005,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
R Kock, and
B Delvoux, and
H Greiling
July 1987,
Clinical chemistry,
R Kock, and
B Delvoux, and
H Greiling
January 2006,
Rapid communications in mass spectrometry : RCM,
R Kock, and
B Delvoux, and
H Greiling
June 1980,
Clinical chemistry,
R Kock, and
B Delvoux, and
H Greiling
August 2013,
Clinical chemistry,
R Kock, and
B Delvoux, and
H Greiling
November 1993,
Biological mass spectrometry,
R Kock, and
B Delvoux, and
H Greiling
January 1981,
Biomedical mass spectrometry,
