BIOMAT Database Logo BIOMAT Database Logo

Integrin expression and collagenase activity of RSV-transformed Syrian hamster fibroblasts, differing in spontaneous metastasizing. 1997

N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russia.

The expression of extracellular matrix (ECM) specific receptors, integrins, and the activities of type IV collagenase and interstitial collagenase were investigated in two strains of oncogenically transformed fibroblasts, drastically differing in spontaneous metastasizing. Both strains were shown to express quite limited patterns of integrins. Of those, alpha 5 beta 1 integrin is greatly reduced on highly metastatic (HM) cells, while the expression of alpha v beta 3 is strongly suppressed on lowly metastatic (LM) fibroblasts. No differences between the strains were found in either intracellular or secreted activities of type IV collagenase, while the activity of interstittial collagenase, secreted by HM cells, was twice as much as secreted by LM cells. The results imply the role of cooperated modifications of integrin-directed cell-ECM interaction and collagenase activity in establishing a metastatic phenotype.

UI MeSH Term Description Entries
D008647 Mesocricetus A genus in the order Rodentia and family Cricetidae. One species, Mesocricetus auratus or golden hamster is widely used in biomedical research. Hamsters, Golden,Hamsters, Golden Syrian,Hamsters, Syrian,Mesocricetus auratus,Syrian Golden Hamster,Syrian Hamster,Golden Hamster,Golden Hamster, Syrian,Golden Hamsters,Golden Syrian Hamsters,Hamster, Golden,Hamster, Syrian,Hamster, Syrian Golden,Syrian Hamsters
D009362 Neoplasm Metastasis The transfer of a neoplasm from one organ or part of the body to another remote from the primary site. Metastase,Metastasis,Metastases, Neoplasm,Metastasis, Neoplasm,Neoplasm Metastases,Metastases
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002461 Cell Line, Transformed Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals. Transformed Cell Line,Cell Lines, Transformed,Transformed Cell Lines
D004789 Enzyme Activation Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme. Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016023 Integrins A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors (RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation. Integrin
D017364 Collagenases Enzymes that catalyze the degradation of collagen by acting on the peptide bonds. Collagen Peptidase,Collagen-Degrading Enzyme,Collagenase,Collagen Degrading Enzyme,Peptidase, Collagen

Related Publications

N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Mutagenicity of methionine or its metabolic products in RSV-transformed Chinese hamster fibroblasts.
January 1987, Anticancer research,
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Regulation of integrin mobility and cytoskeletal association in normal and RSV-transformed chick embryo fibroblasts.
October 1990, Journal of cell science,
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Characterization of stable spontaneous metastatic variant lines of RSV-transformed mouse fibroblasts.
December 1982, International journal of cancer,
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Amplification potential in preneoplastic and neoplastic Syrian hamster embryo fibroblasts transformed by various carcinogens.
July 1993, Cancer research,
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Integrin engagement regulates proliferation and collagenase expression of rheumatoid synovial fibroblasts.
February 1999, Journal of immunology (Baltimore, Md. : 1950),
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Neutral protease activity of Rous sarcoma (RSV) transformed chick embryo fibroblasts.
July 1976, Journal of cell science,
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Uncoupled expression of mRNAs for alpha 1(I) and alpha 2(I) procollagen chains in chemically transformed Syrian hamster fibroblasts.
April 1988, The Journal of biological chemistry,
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Cell/substratum adhesions in RSV-transformed rat fibroblasts.
April 1991, Experimental cell research,
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
Differential role of MAP kinase isoforms in malachite green transformed Syrian hamster embryo fibroblasts in culture.
September 2006, Indian journal of experimental biology,
N I Kozlova, and G E Morozevich, and N I Soloveyva, and S V Vinokurova, and A E Berman
[Resistance-suppressing activity of transformed Syrian hamster cells and their ability to metastasize].
November 1981, Biulleten' eksperimental'noi biologii i meditsiny,
Copied contents to your clipboard!