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A stable-isotope-dilution HPLC-tandem mass spectrometry-based method was developed for the determination of dextromethorphan in human plasma. Plasma samples were prepared for analysis by solid-phase extraction on octadecylsilane extraction cartridges. Dextromethorphan and the deuterium-labeled dextromethorphan internal standard were chromatographed on a short reversed-phase column and detected by a selected-reaction-monitoring scheme. Linear standard curves were obtained over three orders of magnitude and the limit of quantitation for dextromethorphan was 50 pg/ml, using a 1-ml plasma sample. The combination of HPLC and electrospray tandem mass spectrometry resulted in a rapid, selective and sensitive method for the analysis of dextromethorphan in plasma. The method was applied for the evaluation of the pharmacokinetic profile of dextromethorphan in human volunteers following peroral administration.
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
February 2005,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
March 1989,
Biomedical & environmental mass spectrometry,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
January 2006,
Journal of chromatographic science,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
December 2005,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
January 2006,
Rapid communications in mass spectrometry : RCM,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
November 1998,
Journal of chromatography. B, Biomedical sciences and applications,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
October 2006,
Die Pharmazie,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
January 2003,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
November 2012,
Journal of pharmaceutical and biomedical analysis,
T H Eichhold, and
M Quijano, and
W L Seibel, and
C A Cruze, and
R L Dobson, and
K R Wehmeyer
April 2001,
Journal of chromatography. B, Biomedical sciences and applications,
