BIOMAT Database Logo BIOMAT Database Logo

The 3' untranslated region of alfalfa mosaic virus RNA3 contains a core promoter for minus-strand RNA synthesis and an enhancer element. 1997

C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, The Netherlands.

The 3' untranslated regions (UTRs) of the three genomic RNAs of alfalfa mosaic virus consist of a 3' homologous sequence of 145 nt and upstream unique sequences 18-34 nt in length. Mutations were made in the 3' UTR of a cDNA clone of RNA3. Point mutations in five AUGC motifs which interfere with specific binding of coat protein to the 3' UTR had no effect on template activity of RNA3 for minus-strand RNA synthesis in vitro by purified viral RNA-dependent RNA polymerase (RdRp). Deletion analysis showed that the 3' homologous sequence of 145 nt was sufficient for a low level of template activity in the in vitro RdRp assay and a similarly low level of RNA3 accumulation in plants. The presence of an additional sequence of nucleotides 145-165 from the 3' end of RNA3 enhanced template recognition by RdRp in vitro and accumulation of RNA3 in vivo to wild-type levels.

UI MeSH Term Description Entries
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D004742 Enhancer Elements, Genetic Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter. Enhancer Elements,Enhancer Sequences,Element, Enhancer,Element, Genetic Enhancer,Elements, Enhancer,Elements, Genetic Enhancer,Enhancer Element,Enhancer Element, Genetic,Enhancer Sequence,Genetic Enhancer Element,Genetic Enhancer Elements,Sequence, Enhancer,Sequences, Enhancer
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D016679 Genome, Viral The complete genetic complement contained in a DNA or RNA molecule in a virus. Viral Genome,Genomes, Viral,Viral Genomes
D017354 Point Mutation A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair. Mutation, Point,Mutations, Point,Point Mutations
D017794 Alfalfa mosaic virus The type species of the genus ALFAMOVIRUS that is non-persistently transmitted by aphids. Alfalfa mosaic viruses

Related Publications

C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
Recognition of the core RNA promoter for minus-strand RNA synthesis by the replicases of Brome mosaic virus and Cucumber mosaic virus.
November 2000, Journal of virology,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
The 5' untranslated region of alfalfa mosaic virus RNA 1 is involved in negative-strand RNA synthesis.
October 2003, Journal of virology,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
The 3' untranslated region of manganese superoxide dismutase RNA contains a translational enhancer element.
November 1998, Biochemistry,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
A minimal RNA promoter for minus-strand RNA synthesis by the brome mosaic virus polymerase complex.
February 1999, Journal of molecular biology,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
The 3'-untranslated region of alfalfa mosaic virus RNA 3 contains at least two independent binding sites for viral coat protein.
April 1994, Nucleic acids research,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
Maintaining the structural integrity of the Bamboo mosaic virus 3' untranslated region is necessary for retaining the catalytic constant for minus-strand RNA synthesis.
June 2013, Virology journal,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
Role of the Pepino mosaic virus 3'-untranslated region elements in negative-strand RNA synthesis in vitro.
September 2014, Virus research,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
A Y-shaped RNA structure in the 3' untranslated region together with the trans-activator and core promoter of Red clover necrotic mosaic virus RNA2 is required for its negative-strand RNA synthesis.
September 2010, Virology,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
Cellular proteins from human monocytes bind to dengue 4 virus minus-strand 3' untranslated region RNA.
March 2003, Journal of virology,
C M van Rossum, and C B Reusken, and F T Brederode, and J F Bol
The 3'-terminal sequence of Bamboo mosaic virus minus-strand RNA interacts with RNA-dependent RNA polymerase and initiates plus-strand RNA synthesis.
March 2010, Molecular plant pathology,
Copied contents to your clipboard!