Biomaterial Database

Anabolic effect of genistein on bone metabolism in the femoral-metaphyseal tissues of elderly rats is inhibited by the anti-estrogen tamoxifen. 1997

M Yamaguchi, and Y H Gao

Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka City, Japan.

Whether the anabolic effect of genistein on bone metabolism in the femoral-metaphyseal tissues of elderly rats is inhibited by the anti-estrogen tamoxifen was investigated in vitro. Bone tissues were cultured for 24 h in the medium containing either vehicle or genistein. The presence of genistein (10(-6) and 10(-5) M) caused a significant increase in calcium content, alkaline phosphatase activity and DNA content in the bone tissues. The effect of genistein (10(-5) M) in increasing bone alkaline phosphatase activity and DNA content was equal to the stimulatory effect of 17 beta-estradiol (10(-10) and 10(-9) M) on the bone components. The genistein effect was not significantly enhanced by the presence of 17 beta-estradiol (10(-10) and 10(-9) M). The anti-estrogen tamoxifen (10(-7) M) completely blocked the effect of 17 beta-estrogen in increasing bone alkaline phosphatase activity and DNA content. The genistein (10(-5) M)-induced increases in bone alkaline phosphatase activity and DNA content were completely abolished by the presence of tamoxifen (10(-7) and 10(-6) M), although tamoxifen itself had no effect on the bone components. The present study demonstrated that the anabolic effect of genistein on bone components is inhibited by anti-estrogen tamoxifen. The effect of genistein may be mediated through estrogen-like action.

UI	MeSH Term	Description	Entries
D009924	Organ Culture Techniques	A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)	Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D004965	Estrogen Antagonists	Compounds which inhibit or antagonize the action or biosynthesis of estrogenic compounds.	Estradiol Antagonists,Antagonists, Estradiol,Antagonists, Estrogen
D005260	Female		Females
D005269	Femur	The longest and largest bone of the skeleton, it is situated between the hip and the knee.	Trochanter,Greater Trochanter,Lesser Trochanter,Femurs,Greater Trochanters,Lesser Trochanters,Trochanter, Greater,Trochanter, Lesser,Trochanters,Trochanters, Greater,Trochanters, Lesser
D005270	Femur Head	The hemispheric articular surface at the upper extremity of the thigh bone. (Stedman, 26th ed)	Femoral Head,Femoral Heads,Femur Heads,Head, Femoral,Head, Femur
D000375	Aging	The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.	Senescence,Aging, Biological,Biological Aging
D000469	Alkaline Phosphatase	An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013629	Tamoxifen	One of the SELECTIVE ESTROGEN RECEPTOR MODULATORS with tissue-specific activities. Tamoxifen acts as an anti-estrogen (inhibiting agent) in the mammary tissue, but as an estrogen (stimulating agent) in cholesterol metabolism, bone density, and cell proliferation in the ENDOMETRIUM.	ICI-46,474,ICI-46474,ICI-47699,Nolvadex,Novaldex,Soltamox,Tamoxifen Citrate,Tomaxithen,Zitazonium,Citrate, Tamoxifen,ICI 46,474,ICI 46474,ICI 47699,ICI46,474,ICI46474,ICI47699
D017208	Rats, Wistar	A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.	Wistar Rat,Rat, Wistar,Wistar Rats