-transparent.png){kind=logo}
CD5 is useful for the classification of low-grade B-cell lymphomas, because it is aberrantly expressed in B-cell chronic lymphocytic leukemias/small lymphocytic lymphomas (B-CLLs/SLLs) and mantle cell lymphomas (MCLs), but not in the other well-described low-grade B-cell lymphomas. Therefore, we tested the utility of monoclonal antibody (MAb) 4C7 to detect CD5 on paraffin sections of 22 cases with low-grade B-cell lymphomas and 4 cases with high-grade B-cell lymphomas and compared the results with CD5 detection by analysis of fresh tissue with a fluorescent-activated cell sorting (FACS). After heat-induced epitope retrieval we could detect CD5 on paraffin sections in all MCLs and B-CLLs/SLLs, identical to the results of flow cytometry. Eleven of 12 cases that showed no CD5 expression by FACS analysis were also negative on paraffin sections. In only one case of a presumed blastoid MCL, CD5 was detectable by immunohistochemistry but not by FACS analysis. We conclude that MAb 4C7 against CD5 is a useful paraffin-reactive marker, especially to correctly identify MCLs on paraffin sections.
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
October 1993,
The Journal of pathology,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
September 1996,
American journal of clinical pathology,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
February 2013,
American journal of clinical pathology,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
December 2001,
Applied immunohistochemistry & molecular morphology : AIMM,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
December 2000,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
April 1984,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
July 1988,
American journal of clinical pathology,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
March 2005,
Applied immunohistochemistry & molecular morphology : AIMM,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
April 1989,
Journal of the Medical Association of Thailand = Chotmaihet thangphaet,
O Kaufmann, and
B Flath, and
E Späth-Schwalbe, and
K Possinger, and
M Dietel
April 1997,
American journal of clinical pathology,
