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A modified method was compared with an original electrometric method for measurement of erythrocyte acetylcholinesterase (EChE) activity in sheep. The mean +/- SD (pH/30 min) of EChE activity of 8 sheep measured by the modified procedure (0.70 +/- 0.15) was not significantly different from that of the original method (0.64 +/- 0.12). The inherently low plasma cholinesterase activity of the sheep as measured by the 2 methods were also not significantly different from each other (0.09 +/- 0.04 vs 0.10 +/- 0.04). The coefficient of variation of the modified method in measuring EChE activity was 8%. The method was used to demonstrate in vitro inhibition of sheep EChE activity by the organophosphorus and carbamate insecticides dichlorvos and methomyl, respectively. The method could be well-suited for rapid measurement of EChE activity in sheep, especially in cases of organophosphate and possibly carbamate poisoning.
F K Mohammad, and
G A Faris, and
N A al-Kassim
January 2009,
Bulletin of experimental biology and medicine,
F K Mohammad, and
G A Faris, and
N A al-Kassim
January 1983,
Laboratornoe delo,
F K Mohammad, and
G A Faris, and
N A al-Kassim
January 1952,
Helvetica physiologica et pharmacologica acta,
F K Mohammad, and
G A Faris, and
N A al-Kassim
January 1970,
Acta clinica Belgica,
F K Mohammad, and
G A Faris, and
N A al-Kassim
January 1981,
Laboratornoe delo,
F K Mohammad, and
G A Faris, and
N A al-Kassim
January 1964,
Pathologia et microbiologia,
F K Mohammad, and
G A Faris, and
N A al-Kassim
March 1986,
Annals of clinical biochemistry,
F K Mohammad, and
G A Faris, and
N A al-Kassim
April 1982,
Veterinary and human toxicology,
F K Mohammad, and
G A Faris, and
N A al-Kassim
June 1988,
Clinical chemistry,
F K Mohammad, and
G A Faris, and
N A al-Kassim
February 1985,
Archives of neurology,
