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Stable isotope ratio analysis of amino acids: the use of N(O,S)-ethoxycarbonyl trifluoroethyl ester derivatives and gas chromatography/mass spectrometry. 1997
Institute of Clinical Metabolic Research, Medical Faculty Carl Gustav Carus, Technical University Dresden, Germany.
N(O,S)-Ethoxycarbonyl trifluoroethyl amino acid esters are formed by the reaction of amino acids with ethylchloroformate plus trifluoroethanol plus pyridine. The use of these derivatives for a rapid and sensitive determination of specific enrichment of stable isotopically labeled tracer amino acids in blood plasma and protein hydrolysates, by using gas chromatography/electron impact mass spectrometry, is discussed.
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