-transparent.png){kind=logo}
A new approach to detect underivatized carbohydrates in capillary zone electrophoresis (CZE) with a bienzyme electrode is described. The bienzyme electrode is based on immobilization of two enzymes, glucose oxidase and amyloglucosidase, on a platinum microelectrode. Maltose and glucose were used as substrates to establish the method and study its feasibility. Using this electrode, underivatized glucose and maltose were determined after separation in 60 mM borate buffer (pH 9.3) by CZE. A liquid junction, between the surface of the bienzyme electrode and the outlet of the separation capillary, was used to lower the pH of the eluting separation borate buffer so that the immobilized enzymes could maintain high activities. The detection limits were 0.17 mM for maltose and 0.35 mM for glucose. The effects of borate ion and pH on the responses of the bienzyme electrode to maltose and glucose are discussed. The bienzyme electrode did not show any decay in responses to maltose or glucose during one week of continuous operation.
H Wei, and
T Wang, and
S F Li
June 1997,
Electrophoresis,
H Wei, and
T Wang, and
S F Li
October 2011,
Analytical chemistry,
H Wei, and
T Wang, and
S F Li
January 1996,
Journal of chromatography. A,
H Wei, and
T Wang, and
S F Li
May 2007,
Journal of pharmaceutical and biomedical analysis,
H Wei, and
T Wang, and
S F Li
September 1999,
Electrophoresis,
H Wei, and
T Wang, and
S F Li
September 2004,
Talanta,
H Wei, and
T Wang, and
S F Li
May 1996,
Analytical chemistry,
H Wei, and
T Wang, and
S F Li
February 1996,
Electrophoresis,
H Wei, and
T Wang, and
S F Li
June 2003,
Electrophoresis,
H Wei, and
T Wang, and
S F Li
January 1994,
Advances in chromatography,
