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[Changes of lysosomal enzyme activity in the lungs during the course of acute pancreatitis]. 1997

A Anasiewicz, and R Maciejewski, and W Juśkiewicz, and H Lakowska, and B Madej, and P Szkodziak
Katedry i Zakładu Anatomii Prawidłowej Człowieka Akademii Medycznej w Lublinie.

The aim of this work is to study changes in activities of lysosomal enzymes of lungs in Acute Respiratory Distress Syndrome as complication in the course of experimental acute pancreatitis. The study was carried out on 100 male rats, Wistar strain 240-480 g weight. The healthy animals were used as control to find out to norms of activity of lysosomal enzymes of lungs (Cathepsin B, D, L, Acid phosphatase, Lipase, Sulfatase, B-D-galactosidase, N-Acetyl-B-glucosaminidase). The laparotomy was performed on 90 rats and sodium taurocholate (60 animals-OP group) and 0.9% NaCl (30 animals-OK group) was injected pancreaticoduodenal duct. The rats from OP and OK groups were divided into 5 subgroups. After 2, 6, 12, 24 and 48 hours after the operation, animals were sacrificed and the lungs were dissected, weighed, frozen. After thawing the lungs in 0.9% NaCl, 1 g of lungs was sampled at ice melting point. The obtained homogenate centrifuged, supernate was poured out and centrifuged again at a speed of 14,000 rot per min. The activity of enzymes was determined in using supernate preparations by Sigma. Quantitative determination were carried out using Lowrey's method. Differences in values of enzyme activity were statistically significant, between particular groups of animals OK and OP. The highest increase in the activity of enzymes was found between 12 to 24 hours after inducing the disease, what correlated with the increase in volume of lungs. The monitoring of activity of lysosomal enzymes is a sensitive indicator to evaluate the increase of ARDS in the course of experimental acute pancreatitis.

UI MeSH Term Description Entries
D007231 Infant, Newborn An infant during the first 28 days after birth. Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D008168 Lung Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood. Lungs
D008247 Lysosomes A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured or undergoes MEMBRANE FUSION. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed). Autolysosome,Autolysosomes,Lysosome
D008297 Male Males
D010195 Pancreatitis INFLAMMATION of the PANCREAS. Pancreatitis is classified as acute unless there are computed tomographic or endoscopic retrograde cholangiopancreatographic findings of CHRONIC PANCREATITIS (International Symposium on Acute Pancreatitis, Atlanta, 1992). The two most common forms of acute pancreatitis are ALCOHOLIC PANCREATITIS and gallstone pancreatitis. Acute Edematous Pancreatitis,Acute Pancreatitis,Pancreatic Parenchyma with Edema,Pancreatic Parenchymal Edema,Pancreatitis, Acute,Pancreatitis, Acute Edematous,Peripancreatic Fat Necrosis,Acute Edematous Pancreatitides,Acute Pancreatitides,Edema, Pancreatic Parenchymal,Edematous Pancreatitides, Acute,Edematous Pancreatitis, Acute,Fat Necrosis, Peripancreatic,Necrosis, Peripancreatic Fat,Pancreatic Parenchymal Edemas,Pancreatitides, Acute,Pancreatitides, Acute Edematous,Parenchymal Edema, Pancreatic,Peripancreatic Fat Necroses
D012128 Respiratory Distress Syndrome A syndrome characterized by progressive life-threatening RESPIRATORY INSUFFICIENCY in the absence of known LUNG DISEASES, usually following a systemic insult such as surgery or major TRAUMA. ARDS, Human,Acute Respiratory Distress Syndrome,Adult Respiratory Distress Syndrome,Pediatric Respiratory Distress Syndrome,Respiratory Distress Syndrome, Acute,Respiratory Distress Syndrome, Adult,Respiratory Distress Syndrome, Pediatric,Shock Lung,Distress Syndrome, Respiratory,Distress Syndromes, Respiratory,Human ARDS,Lung, Shock,Respiratory Distress Syndromes,Syndrome, Respiratory Distress
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006867 Hydrolases Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3. Hydrolase
D000208 Acute Disease Disease having a short and relatively severe course. Acute Diseases,Disease, Acute,Diseases, Acute
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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