-transparent.png){kind=logo}
In this paper, we have characterized the regulation of plasmin activity by annexin II tetramer (AIIt). Plasmin activity was measured by a fibrin lysis assay in which a fibrin polymer was produced from purified components and the extent of polymer lysis was determined by following changes in turbidity. Extrinsic lysis of the fibrin polymer, initiated by addition of tissue plasminogen activator (t-PA), was totally blocked if AIIt was present during fibrin polymer formation. Furthermore, fibrin polymer formed in the presence of AIIt was resistant to extrinsic lysis initiated by addition of plasmin. AIIt bound to fibrin polymer under conditions in which polymer lysis was inhibited. Plasmin-dependent extrinsic lysis of the fibrin polymer was also blocked if AIIt was present in the incubation medium, and under these conditions the amidolytic activity of plasmin, measured with an artificial substrate, was inhibited about 5-fold. In contrast, in the absence of fibrin, and at an AIIt/plasmin molar ratio of 526, the amidolytic activity of plasmin was inhibited by only 22.3% +/- 7.4% (mean +/- SD, n = 5) by AIIt. Plasmin-dependent fibrinolysis was only slightly inhibited if fibrin polymer was formed in the presence of annexins I, II, V, or VI. These results identify AIIt as an in vitro regulator of plasmin activity.
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
February 2000,
Biochemistry,
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
March 2000,
Biochemistry,
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
January 2002,
Methods in molecular biology (Clifton, N.J.),
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
January 2002,
Biochemistry,
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
January 1995,
Molecular and cellular biochemistry,
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
July 2001,
The Journal of biological chemistry,
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
November 2009,
The Journal of clinical investigation,
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
September 2000,
Archives of biochemistry and biophysics,
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
November 1995,
Biochemistry,
K S Choi, and
J Ghuman, and
G Kassam, and
H M Kang, and
S L Fitzpatrick, and
D M Waisman
January 1999,
Trends in cardiovascular medicine,
