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The effect of estradiol-17-beta treatment on phosvitin synthesis by cultured chick embryo liver cells has been studied. Phosvitin synthesis occurs approximately 15 hr of hormone treatment; the synthesis being blocked by actinomycin D treatment suggests that RNA synthesis is required. The life time of the newly synthesized RNA is at least 24 hr. The significance of these findings with respect to the mechanisms involved in hormone-mediated protein synthesis is discussed.
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