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A rapid amplification of cDNA ends method, using degenerate oligonucleotides based upon the N-terminal amino acid sequence of human hepatic deoxyribonuclease II (DNase II), allowed a novel cDNA encoding DNase II to be constructed from thyroid gland RNA. The composite nucleotide sequence (1593 bases) included an open reading frame of 1080 bases, which encoded a single polypeptide of 360 amino acids (signal peptide, 16; propeptide, 91; mature protein, 253). Although the sequence of DNase II showed no significant homology to other mammalian proteins, its cDNA structural organization resembled those of the lysosomal cathepsin families. The two parts of the cDNA corresponding to the propeptide and the mature protein were expressed in Escherichia coli, and the recombinant polypeptides thus obtained were strongly stained with an anti-DNase II antibody on Western blotting. DNase II is ubiquitously expressed in human tissues, and the DNase II gene (DNASE2) was assigned to chromosome 19.
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
October 1995,
Biological & pharmaceutical bulletin,
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
November 1993,
Biochimica et biophysica acta,
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
May 1991,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
January 1984,
Nature,
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
October 1995,
DNA and cell biology,
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
January 1984,
Nature,
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
June 1991,
Matrix (Stuttgart, Germany),
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
July 1998,
The Journal of biological chemistry,
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
December 1999,
Glycoconjugate journal,
T Yasuda, and
H Takeshita, and
R Iida, and
T Nakajima, and
O Hosomi, and
Y Nakashima, and
K Kishi
July 1988,
Proceedings of the National Academy of Sciences of the United States of America,
