-transparent.png){kind=logo}
We have used the buoyant density shift method to measure excision-repair patch lengths in UV-irradiated repair-proficient human cells and in primary fibroblasts belonging to xeroderma pigmentosum complementation group C (XP-C), in which excision repair of UV-induced photoproducts is dependent upon transcription. The patch size was found to be about 30 nucleotides for both cell types. This agrees with the size of the DNA fragments excised in vitro by the dual incisions of the structure-specific nucleases XPG and ERCC1-XPF. We conclude that the XPC protein is not required to target the excision nucleases to sites of DNA cleavage in transcribed strands of expressed genes or to protect the newly incised DNA from further processing by exonucleases.
K K Bowman, and
C A Smith, and
P C Hanawalt
December 2001,
Journal of photochemistry and photobiology. B, Biology,
K K Bowman, and
C A Smith, and
P C Hanawalt
May 2015,
Genes & development,
K K Bowman, and
C A Smith, and
P C Hanawalt
January 2023,
Journal of biosciences,
K K Bowman, and
C A Smith, and
P C Hanawalt
November 1981,
Journal of virology,
K K Bowman, and
C A Smith, and
P C Hanawalt
December 1999,
Proceedings of the National Academy of Sciences of the United States of America,
K K Bowman, and
C A Smith, and
P C Hanawalt
June 2003,
Proceedings of the National Academy of Sciences of the United States of America,
K K Bowman, and
C A Smith, and
P C Hanawalt
October 1997,
Nucleic acids research,
K K Bowman, and
C A Smith, and
P C Hanawalt
November 1999,
Oncogene,
K K Bowman, and
C A Smith, and
P C Hanawalt
May 2010,
PLoS genetics,
K K Bowman, and
C A Smith, and
P C Hanawalt
July 2004,
DNA repair,
