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CENP-B has been suggested to organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. The N-terminal portion of CENP-B is a 15 kDa DNA binding domain (DBD) consisting of two repeating units, RP1 and RP2. The DBD specifically binds to the CENP-B box sequence (17 bp) in centromere DNA. We determined the solution structure of human CENP-B DBD RP1 by multi-dimensional 1H, 13C and 15N NMR methods. The CENP-B DBD RP1 structure consists of four helices and has a helix-turn-helix structure. The overall folding is similar to those of some other eukaryotic DBDs, although significant sequence homology with these proteins was not found. The DBD of yeast RAP1, a telomere binding protein, is most similar to CENP-B DBD RP1. We studied the interaction between CENP-B DBD RP1 and the CENP-B box by the use of NMR chemical shift perturbation. The results suggest that CENP-B DBD RP1 interacts with one of the essential regions of the CENP-B box DNA, mainly at the N-terminal basic region, the N-terminal portion of helix 2 and helix 3.
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
December 2003,
The Journal of biological chemistry,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
April 1992,
Journal of biochemistry,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
May 1990,
The Journal of cell biology,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
July 1991,
Chromosoma,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
January 1991,
Acta histochemica. Supplementband,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
July 1992,
Bioscience, biotechnology, and biochemistry,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
September 1996,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
November 2019,
Nucleic acids research,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
March 2005,
Seikagaku. The Journal of Japanese Biochemical Society,
J Iwahara, and
T Kigawa, and
K Kitagawa, and
H Masumoto, and
T Okazaki, and
S Yokoyama
March 1998,
The Journal of rheumatology,
