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OBJECTIVE This study investigates heat shock protein 70 (HSP70) expression by peripheral blood mononuclear cells (PBMCs) of septic patients admitted to an intensive care unit and examines the possibility of a correlation between HSP70 levels and plasma tumor necrosis factor alpha (TNF-alpha) concentrations. Additionally, we evaluated whether the HSP70 production could be regarded as a prognostic factor for the development of septic shock as well as for patient survival. METHODS Blood samples of 29 patients were taken 24 hours after the diagnosis of sepsis. HSP70 expression and TNF-alpha level were measured using indirect immunofluorescent analysis and a commercially available enzyme-linked immunosorbent assay method, respectively. RESULTS PBMCs expressed significantly high levels of HSP70 (11.9 +/- 5.6 [sd]) compared with those of the healthy control group (3.2 +/- 2.1% positive cells). Such enhanced levels were correlated to plasma TNF-alpha concentrations (r = .99, P < .01). This study failed to demonstrate a relationship between HSP70 production and clinical outcome. CONCLUSIONS These findings give further evidence that also in humans, heat shock response is activated during sepsis. The correlation observed between HSP70 overproduction and TNF-alpha plasma concentrations suggests that HSP70 exerts a possible protective effect against TNF-alpha cytotoxicity. Such hypothesis has not been confirmed by our clinical data.
G Delogu, and
L Lo Bosco, and
M Marandola, and
G Famularo, and
L Lenti, and
F Ippoliti, and
L Signore
August 1999,
Nihon rinsho. Japanese journal of clinical medicine,
G Delogu, and
L Lo Bosco, and
M Marandola, and
G Famularo, and
L Lenti, and
F Ippoliti, and
L Signore
February 1989,
The Journal of biological chemistry,
G Delogu, and
L Lo Bosco, and
M Marandola, and
G Famularo, and
L Lenti, and
F Ippoliti, and
L Signore
September 2020,
Bulletin of experimental biology and medicine,
G Delogu, and
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January 1994,
Archives of dermatological research,
G Delogu, and
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G Famularo, and
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August 1996,
Experimental cell research,
G Delogu, and
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November 2013,
Journal of psychiatric research,
G Delogu, and
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September 1999,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
G Delogu, and
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G Famularo, and
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L Signore
January 2007,
Nutrition (Burbank, Los Angeles County, Calif.),
G Delogu, and
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M Marandola, and
G Famularo, and
L Lenti, and
F Ippoliti, and
L Signore
May 2005,
Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies,
G Delogu, and
L Lo Bosco, and
M Marandola, and
G Famularo, and
L Lenti, and
F Ippoliti, and
L Signore
August 2007,
Marine environmental research,
