BIOMAT Database Logo BIOMAT Database Logo

Adenosine A1 receptor-mediated modulation of dopamine D1 receptors in stably cotransfected fibroblast cells. 1998

S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Department of Neuroscience, Karolinska Institute, S-171 77 Stockholm, Sweden.

The antagonistic interactions between adenosine A1 and dopamine D1 receptors were studied in a mouse Ltk- cell line stably cotransfected with human adenosine A1 receptor and dopamine D1 receptor cDNAs. In membrane preparations, both the adenosine A1 receptor agonist N6-cyclopentyladenosine and the GTP analogue guanyl-5'-yl imidodiphospate induced a decrease in the proportion of dopamine D1 receptors in a high affinity state. In the cotransfected cells, the adenosine A1 agonist induced a concentration-dependent inhibition of dopamine-induced cAMP accumulation. Blockade of adenosine A1 receptor signal transduction with the adenosine A1 receptor antagonist 1,3-dipropyl-8-cyclopentylxanthine or with pertussis toxin pretreatment increased both basal and dopamine-stimulated cAMP levels, indicating the existence of tonic adenosine A1 receptor activation. Pretreatment with pertussis toxin also counteracted the effects of low concentrations of the A1 agonist on D1 receptor-agonist binding. The results suggest that adenosine A1 receptors antagonistically modulate dopamine D1 receptors at the level of receptor binding and the generation of second messengers.

UI MeSH Term Description Entries
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004298 Dopamine One of the catecholamine NEUROTRANSMITTERS in the brain. It is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action. Hydroxytyramine,3,4-Dihydroxyphenethylamine,4-(2-Aminoethyl)-1,2-benzenediol,Dopamine Hydrochloride,Intropin,3,4 Dihydroxyphenethylamine,Hydrochloride, Dopamine
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001552 Benzazepines Compounds with BENZENE fused to AZEPINES.
D001667 Binding, Competitive The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements. Competitive Binding
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D014316 Tritium The radioactive isotope of hydrogen also known as hydrogen-3. It contains two NEUTRONS and one PROTON in its nucleus and decays to produce low energy BETA PARTICLES. Hydrogen-3,Hydrogen 3
D014970 Xanthines Purine bases found in body tissues and fluids and in some plants.

Related Publications

S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Activation of adenosine A1 receptor modulates dopamine D1 receptor activity in stably cotransfected human embryonic kidney 293 cells.
October 2006, European journal of pharmacology,
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Adenosine A2A receptors modulate the binding characteristics of dopamine D2 receptors in stably cotransfected fibroblast cells.
December 1996, European journal of pharmacology,
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Modulation of dopamine D1 receptor signaling by adenosine A1 receptors in Sf9 cells requires expression of Gi proteins.
October 2006, Neuroscience letters,
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Role of adenosine A1 receptors in the modulation of dopamine D1 and adenosine A2A receptor signaling in the neostriatum.
August 2006, Neuroscience,
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Adenosine A1 receptor-dopamine D1 receptor interaction in the rat limbic system: modulation of dopamine D1 receptor antagonist binding sites.
April 1996, Neuroscience letters,
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Adenosine A1 receptor-mediated inhibition of dopamine release from rat striatal slices is modulated by D1 dopamine receptors.
December 2007, The European journal of neuroscience,
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Receptor-receptor interactions involving adenosine A1 or dopamine D1 receptors and accessory proteins.
January 2007, Journal of neural transmission (Vienna, Austria : 1996),
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Dopamine D1 receptor-mediated facilitation of GABAergic neurotransmission in the rat strioentopenduncular pathway and its modulation by adenosine A1 receptor-mediated mechanisms.
July 1996, The European journal of neuroscience,
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Activation of adenosine A1 and A2A receptors modulates dopamine D2 receptor-induced responses in stably transfected human neuroblastoma cells.
January 2000, Journal of neurochemistry,
S Ferré, and M Torvinen, and K Antoniou, and E Irenius, and O Civelli, and E Arenas, and B B Fredholm, and K Fuxe
Postsynaptic antagonistic interaction between adenosine A1 and dopamine D1 receptors.
December 1994, Neuroreport,
Copied contents to your clipboard!