-transparent.png){kind=logo}
A new species of DNA polymerase has been purified more than 10 000-fold from the cytoplasm of erythroid hyperplastic bone marrow. This DNA polymerase, in contrast to previously described eukaryotic DNA polymerases, is associated with a very active 3' to 5' exonuclease activity. Similar to the 3' to 5' exonuclease activity associated with prokaryotic DNA polymerases, this enzyme catalyzes the removal of 3'-terminal nucleotides from DNA, as well as a template-dependent conversion of deoxyribonucleoside triphosphates to monophosphates. The exonuclease activity is not separable from the DNA polymerase activity by chromatography on DEAE-Sephadex or hydroxylapatite, and upon sucrose density gradient centrifugation the two activities cosediment at 7 S or at 11 S depending on the ionic strength. Both exonuclease and polymerase activities have identical rates of heat inactivation and both are equally sensitive to hemin and Rifamycin AF/013, inhibitors of DNA synthesis that act by binding to DNA polymerase and causing its dissociation from its template/primer. These results are consistent with the coexistence of two enzyme activities in a single protein.
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
April 1988,
Biochemistry,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
September 1984,
Biochemical and biophysical research communications,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
April 1993,
Biochemical pharmacology,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
May 1991,
Virology,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
October 1992,
Proceedings of the National Academy of Sciences of the United States of America,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
March 1996,
Genetic analysis : biomolecular engineering,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
February 2002,
TheScientificWorldJournal,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
July 1981,
Biochimica et biophysica acta,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
January 1983,
Doklady Akademii nauk SSSR,
J J Byrnes, and
K M Downey, and
V L Black, and
A G So
December 1989,
The Journal of biological chemistry,
