BIOMAT Database Logo BIOMAT Database Logo

[Isolation of a cellular receptor for tick-borne encephalitis virus using anti-idiotypic antibodies]. 1997

E V Protopopova, and S N Konavalova, and V B Loktev

Cell surface receptors for tick-borne encephalitis (TBE) virus are proposed to be identified by affinity chromatography with polyclonal antiidiotypic antibodies (AIA) as antireceptor antibodies. A single affinity chromatography procedure permitted us to obtain 33-150 times purified cell surface receptor from human embryonic renal cell membranes. Four polypeptides with molecular weight 43, 67, 110, and 210 kD were detected in highly purified fractions of TBE virus cell surface receptor. Antireceptor antibodies reacted in immunoblotting only with the 67 kD polypeptide. The molecular weight of protein p67 suggests that it is the nonintegrin laminin receptor. Monoclonal antibodies 8E3 to beta 1-chain of human integrin and M-KID2 to alpha 3 beta 1-integrin reacted in enzyme immunoassay and spot analysis with purified cell surface receptor for TBE virus. The molecular weight of beta 1-chain of human integrin is 110 kD. Probably, the 210 kD polypeptide is an alpha 3-chain of human integrin. Laminin serves as the ligand for alpha 3 beta 1-integrin and laminin receptor, which can be the structural basis for joint isolation of these molecules from lysates of RH cell membranes in a column with immobilized antireceptor antibodies. The role of p43 protein is not clear. Identification of two laminin-binding protein molecules by means of antireceptor antibodies permits us to propose that laminin-binding sites of human alpha 3 beta 1-integrin and laminin receptor can ensure the receptor interactions between TBE virions and RH cells.

UI MeSH Term Description Entries
D011991 Receptors, Virus Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response. Viral Entry Receptor,Viral Entry Receptors,Virus Attachment Factor,Virus Attachment Factors,Virus Attachment Receptor,Virus Attachment Receptors,Virus Entry Receptor,Virus Entry Receptors,Virus Receptor,Virus Receptors,Attachment Factor, Virus,Attachment Factors, Virus,Attachment Receptor, Virus,Attachment Receptors, Virus,Entry Receptor, Viral,Entry Receptor, Virus,Entry Receptors, Viral,Entry Receptors, Virus,Receptor, Viral Entry,Receptor, Virus,Receptor, Virus Attachment,Receptor, Virus Entry,Receptors, Viral Entry,Receptors, Virus Attachment,Receptors, Virus Entry
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D004669 Encephalitis Viruses, Tick-Borne A subgroup of the genus FLAVIVIRUS that causes encephalitis and hemorrhagic fevers and is found in eastern and western Europe and the former Soviet Union. It is transmitted by TICKS and there is an associated milk-borne transmission from viremic cattle, goats, and sheep. Hemorrhagic Fever Virus, Omsk,Kyasanur Forest disease virus,Langat virus,Louping ill virus,Omsk hemorrhagic fever virus,Powassan virus,Al-Khurma Hemorrhagic Fever Virus,Al-Khurma virus,Al-Khurma virus (ALKV),Alkhurma Hemorrhagic Fever Virus,Alkhurma virus,Alkhurma virus (ALKV),Encephalitis Virus, Tick-Borne,Tick-Borne Encephalitis Virus,Tick-Borne Encephalitis Viruses,Viruses, Tick-Borne Encephalitis,Al Khurma Hemorrhagic Fever Virus,Al Khurma virus,Al Khurma virus (ALKV),Encephalitis Virus, Tick Borne,Encephalitis Viruses, Tick Borne,Louping ill viruses,Tick Borne Encephalitis Virus,Tick Borne Encephalitis Viruses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000888 Antibodies, Anti-Idiotypic Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies. Anti-Antibodies,Anti-Idiotype Antibodies,Antibodies, Internal Image,Antigamma Globulin Antibodies,Antiglobulins,Anti Antibodies,Anti-gamma Globulin Antibodies,Anti Idiotype Antibodies,Anti gamma Globulin Antibodies,Anti-Idiotypic Antibodies,Antibodies, Anti,Antibodies, Anti Idiotypic,Antibodies, Anti-Idiotype,Antibodies, Anti-gamma Globulin,Antibodies, Antigamma Globulin,Globulin Antibodies, Anti-gamma,Globulin Antibodies, Antigamma,Image Antibodies, Internal,Internal Image Antibodies
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings

Related Publications

E V Protopopova, and S N Konavalova, and V B Loktev
A putative host cell receptor for tick-borne encephalitis virus identified by anti-idiotypic antibodies and virus affinoblotting.
January 1999, Intervirology,
E V Protopopova, and S N Konavalova, and V B Loktev
[The discovery of a possible cell receptor for the tick-borne encephalitis virus (TBE) by using anti-idiotypic antibodies to viral protein E].
January 1990, Doklady Akademii nauk SSSR,
E V Protopopova, and S N Konavalova, and V B Loktev
Induction and characterization of monoclonal anti-idiotypic antibodies to tick-borne encephalitis virus neutralizing antibody.
November 1995, Journal of immunoassay,
E V Protopopova, and S N Konavalova, and V B Loktev
Chimeric antibodies against tick-borne encephalitis virus.
July 2010, Vaccine,
E V Protopopova, and S N Konavalova, and V B Loktev
[Recombinant antibodies to tick-borne encephalitis virus].
January 2002, Voprosy virusologii,
E V Protopopova, and S N Konavalova, and V B Loktev
[ISOLATION OF TICK-BORNE ENCEPHALITIS VIRUS FROM JAYS].
January 1965, Meditsinskaia parazitologiia i parazitarnye bolezni,
E V Protopopova, and S N Konavalova, and V B Loktev
[Preparation and study of properties of anti-idiotypic antibodies, carrying hemagglutinating paratopes of tick-borne encephalitis virus on their surface].
January 1996, Voprosy virusologii,
E V Protopopova, and S N Konavalova, and V B Loktev
[Isolation of tick-borne encephalitis virus from Oricichla dauma].
January 1962, Voprosy virusologii,
E V Protopopova, and S N Konavalova, and V B Loktev
Prevalence of tick-borne-encephalitis virus antibodies in Lithuania.
July 2002, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology,
E V Protopopova, and S N Konavalova, and V B Loktev
Tick-borne encephalitis virus.
May 2022, Journal of medical microbiology,
Copied contents to your clipboard!