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The synthetic formylpeptide fMLP is widely used as a model chemoattractant and secretagogue for mammalian neutrophils. Despite possessing fMLP receptors, equine neutrophils do not produce superoxide anions in response to fMLP and there is no inflammatory reaction in the horse when fMLP is injected intradermally. The functional capability of these receptors was investigated after pretreatment with recognized priming agents. Purified neutrophils were pretreated with lipopolysaccharide (LPS), platelet-activating factor (PAF), or tumor necrosis factor alpha (TNF-alpha) and superoxide anion generation and shape change quantified by lucigenin-dependent chemiluminescence (LDCL) and flow cytometry, respectively. LPS, TNF-alpha, and PAF pretreatment induced significant LDCL in response to fMLP; similarly LPS pretreatment was a prerequisite for fMLP-stimulated neutrophil polarization in response to fMLP. However, LPS failed to induce fMLP-mediated chemotaxis of equine neutrophils. These data indicate that equine neutrophil fMLP receptors are not vestigial as previously thought but can trigger both respiratory burst activity and cell polarization responses after priming.
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
October 1990,
American journal of veterinary research,
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
October 1994,
Journal of biomedical science,
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
March 1989,
Agents and actions,
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
November 1989,
Blood,
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
June 1986,
Pediatric research,
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
October 1983,
Blood,
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
September 1983,
FEBS letters,
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
December 1988,
Journal of immunology (Baltimore, Md. : 1950),
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
July 1992,
Agents and actions,
T J Brazil, and
A G Rossi, and
C Haslett, and
B McGorum, and
P M Dixon, and
E R Chilvers
September 1989,
Gastroenterology,
