Amino acid changes in the coat protein of cucumber mosaic virus differentially affect transmission by the aphids Myzus persicae and Aphis gossypii. 1998

K L Perry, and L Zhang, and P Palukaitis
Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907, USA. perry@btny.purdue.edu

Cucumber mosaic virus (CMV) transmission by two aphid species was investigated using strains of the virus with modified coat proteins. Chimeric viral RNA 3 cDNA constructs were designed with coat protein-encoding sequences from the efficiently aphid-transmitted Fny-CMV and the very poorly aphid-transmitted M-CMV. Different regions of the viral coat protein variably influenced transmission; the magnitude of effects depended on the aphid species used as vector. Five amino acid changes in the coat protein of M-CMV (positions 25, 129, 162, 168, and 214) were required to restore efficient transmission by Myzus persicae. This contrasts with previously demonstrated, more limited changes required for restoration of transmission by Aphis gossypii. A construct with modified amino acids 129, 162, and 168 was efficiently transmitted by A. gossypii, while remaining poorly transmissible by M. persicae. Transmission of Fny-CMV by single aphids of A. gossypii and M. persicae was 54.0% and 27.9%, respectively. Single coat protein amino acid changes in Fny-CMV dramatically reduced transmission by M. persicae, while showing little effect on transmission by A. gossypii.

UI MeSH Term Description Entries
D010935 Plant Diseases Diseases of plants. Disease, Plant,Diseases, Plant,Plant Disease
D011993 Recombinant Fusion Proteins Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes. Fusion Proteins, Recombinant,Recombinant Chimeric Protein,Recombinant Fusion Protein,Recombinant Hybrid Protein,Chimeric Proteins, Recombinant,Hybrid Proteins, Recombinant,Recombinant Chimeric Proteins,Recombinant Hybrid Proteins,Chimeric Protein, Recombinant,Fusion Protein, Recombinant,Hybrid Protein, Recombinant,Protein, Recombinant Chimeric,Protein, Recombinant Fusion,Protein, Recombinant Hybrid,Proteins, Recombinant Chimeric,Proteins, Recombinant Fusion,Proteins, Recombinant Hybrid
D002213 Capsid The outer protein protective shell of a virus, which protects the viral nucleic acid. Capsids are composed of repeating units (capsomers or capsomeres) of CAPSID PROTEINS which when assembled together form either an icosahedral or helical shape. Procapsid,Prohead,Capsids,Procapsids,Proheads
D002678 Chimera An individual that contains cell populations derived from different zygotes. Hybrids,Chimeras,Hybrid
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001042 Aphids A family (Aphididae) of small insects, in the suborder Sternorrhyncha, that suck the juices of plants. Important genera include Schizaphis and Myzus. The latter is known to carry more than 100 virus diseases between plants. Aphididae,Lice, Plant,Myzus,Plant Lice,Schizaphis,Louse, Plant,Plant Louse,Aphid,Lices, Plant,Louses, Plant,Plant Lices,Plant Louses,Schizaphi
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D016297 Mutagenesis, Site-Directed Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion. Mutagenesis, Oligonucleotide-Directed,Mutagenesis, Site-Specific,Oligonucleotide-Directed Mutagenesis,Site-Directed Mutagenesis,Site-Specific Mutagenesis,Mutageneses, Oligonucleotide-Directed,Mutageneses, Site-Directed,Mutageneses, Site-Specific,Mutagenesis, Oligonucleotide Directed,Mutagenesis, Site Directed,Mutagenesis, Site Specific,Oligonucleotide Directed Mutagenesis,Oligonucleotide-Directed Mutageneses,Site Directed Mutagenesis,Site Specific Mutagenesis,Site-Directed Mutageneses,Site-Specific Mutageneses
D017354 Point Mutation A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair. Mutation, Point,Mutations, Point,Point Mutations
D017799 Cucumovirus A genus of plant viruses of the family BROMOVIRIDAE, which infect cucurbits and solanaceous plants. Transmission occurs via aphids in a non-persistent manner, and also via seeds. The type species Cucumber mosaic virus, a CUCUMOVIRUS, should not be confused with Cucumber green mottle mosaic virus, a TOBAMOVIRUS. Cucumber mosaic virus,Cucumber mosaic viruses,Cucumoviruses

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