BIOMAT Database Logo BIOMAT Database Logo

Cloning and sequencing of the Sphingomonas (Pseudomonas) paucimobilis gene essential for the O demethylation of vanillate and syringate. 1998

S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
New Products & Technology Laboratory, Cosmo Research Institute, Saitama, Japan. seiji-nk@ja2.so-net.or.jp

Sphingomonas (Pseudomonas) paucimobilis SYK-6 is able to grow on 5,5'-dehydrodivanillic acid (DDVA), syringate, vanillate, and other dimeric model compounds of lignin as a sole carbon source. Nitrosoguanidine mutagenesis of S. paucimobilis SYK-6 was performed, and two mutants with altered DDVA degradation pathways were isolated. The mutant strain NT-1 could not degrade DDVA, but could degrade syringate, vanillate, and 2,2',3'-trihydroxy-3-methoxy-5,5'-dicarboxybiphenyl (OH-DDVA). Strain DC-49 could slowly assimilate DDVA, but could degrade neither vanillate nor syringate, although it could degrade protocatechuate and 3-O-methylgallate. A complementing DNA fragment of strain DC-49 was isolated from the cosmid library of strain SYK-6. The minimum DNA fragment complementing DC-49 was determined to be the 1.8-kbp insert of pKEX2.0. Sequencing analysis showed an open reading frame of 1,671 bp in this fragment, and a similarity search indicated that the deduced amino acid sequence of this open reading frame had significant similarity (60%) to the formyltetrahydrofolate synthetase of Clostridium thermoaceticum.

UI MeSH Term Description Entries
D008031 Lignin The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed) Lignins
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011549 Pseudomonas A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants. Chryseomonas,Pseudomona,Flavimonas
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003640 Dealkylation The removing of alkyl groups from a compound. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed) Dealkylations
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D005707 Gallic Acid A colorless or slightly yellow crystalline compound obtained from nutgalls. It is used in photography, pharmaceuticals, and as an analytical reagent. 3,4,5-Trihydroxybenzoic Acid,Acid, Gallic
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D014641 Vanillic Acid A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13). 4-Hydroxy-3-methoxybenzoic Acid,p-Hydroxy-m-methoxy-benzoic Acid,4 Hydroxy 3 methoxybenzoic Acid,Acid, 4-Hydroxy-3-methoxybenzoic,Acid, Vanillic,Acid, p-Hydroxy-m-methoxy-benzoic,p Hydroxy m methoxy benzoic Acid

Related Publications

S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6.
March 2005, Journal of bacteriology,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
A novel tetrahydrofolate-dependent O-demethylase gene is essential for growth of Sphingomonas paucimobilis SYK-6 with syringate.
May 2004, Journal of bacteriology,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6.
December 2000, Journal of bacteriology,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
Coexistence of two different O demethylation systems in lignin metabolism by Sphingomonas paucimobilis SYK-6: cloning and sequencing of the lignin biphenyl-specific O-demethylase (LigX) gene.
May 2000, Applied and environmental microbiology,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
Vanillate and syringate cements.
January 1988, Trends & techniques in the contemporary dental laboratory,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase.
October 1988, Journal of bacteriology,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
Enzymatic Kraft lignin demethylation and fungal O-demethylases like vanillate-O-demethylase and syringate O-demethylase catalyzed catechol-Fe3+ complexation method.
September 2018, Journal of microbiological methods,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
[Bacteremia caused by Sphingomonas (Pseudomonas) paucimobilis].
October 1998, Enfermedades infecciosas y microbiologia clinica,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
Cloning and sequencing of the gene for a Pseudomonas paucimobilis enzyme that cleaves beta-aryl ether.
December 1991, Journal of bacteriology,
S Nishikawa, and T Sonoki, and T Kasahara, and T Obi, and S Kubota, and S Kawai, and N Morohoshi, and Y Katayama
Cloning and sequence analysis of the ces10 gene encoding a Sphingomonas paucimobilis esterase.
June 2003, Applied microbiology and biotechnology,
Copied contents to your clipboard!