Biomaterial Database

Studies on cyclodextrin glycosyltransferase. IV. Enzymatic synthesis of 3-O-alpha-D-glucopyranosyl-L-sorbose and 4-O-alpha-D-glucopyranosyl-D-xylose using cyclodextrin glycosyltransferase. 1976

S Kitahata, and S Okada

The acceptor specificity of the transglycosylation reaction of cyclodextrin glycosyltransferase[EC 2.4.1.19] was investigated using various sugars and sugar alcohols. L-Sorbose, D-xylose, and D-galactose, which contain configurational or structural changes relative to the D-glucopyranose unit at positions other than position 1, were also shown to be efficient acceptors in the transglycosylation reaction of this enzyme. It was shown by chemical and enzymatic methods that this enzyme could transfer glycosyl residues only to the C3-hydroxyl group of L-sorbose and C4-hydroxyl group of D-xylose, producing oligosaccharides terminated by 3-O-alpha-D-glucopyranosyl-L-sorbose and 4-O-alpha-D-glucopyranosyl-D-xylose at the reducing ends, respectively.

UI	MeSH Term	Description	Entries
D007854	Lead	A soft, grayish metal with poisonous salts; atomic number 82, atomic weight 207.2, symbol Pb.
D009844	Oligosaccharides	Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.	Oligosaccharide
D005690	Galactose	An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.	D-Galactose,Galactopyranose,Galactopyranoside,D Galactose
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D005964	Glucosyltransferases	Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.	Glucosyltransferase
D000085	Acetates	Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.	Acetate,Acetic Acid Esters,Acetic Acids,Acids, Acetic,Esters, Acetic Acid
D001407	Bacillus	A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.	Bacillus bacterium
D013013	Sorbose	A ketose sugar that is commonly used in the commercial synthesis of ASCORBIC ACID.	D-Sorbose,L-Sorbose,D Sorbose,L Sorbose
D013329	Structure-Activity Relationship	The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.	Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships
D014994	Xylose		D-Xylose,D Xylose