Characterization of a murine monoclonal antibody specific for swine beta1 integrin. 1998

C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
Institut de Transplantation et de Recherche en Transplantation, Institut National de la Santé et de la Recherche Médicale, Unité 437, Nantes, France.

Murine monoclonal antibodies were raised against porcine platelets in order to provide tools for investigating interactions of human blood cells and natural antibodies with porcine tissues. Hybridomas were screened by cellular ELISA on porcine platelets and endothelial cells. Positive clones were tested by flow cytometry for reactivity with isolated endothelial cells. One clone, NaM160-1A3, produced an antibody that stained porcine but not human endothelial cells and lymphocytes. The antibody bound to a 116 kDa glycoprotein on Western blot of both platelets and endothelial cells. The antigen was purified from a platelet lysate by affinity chromatography, first on a ConA column and then on a column presenting the immobilized NaM160-1A3 antibody. Two glycoproteins were obtained: one (116 kDa) was recognized by the antibody and one (150 kDa) was not. The 116 kDa protein had an internal decapeptide identical with human beta 1 integrin, and the 150 kDa protein had an internal amino acid sequence belonging to porcine alpha 2 integrin. Therefore, the NaM160-1A3 antibody was directed against porcine beta 1 integrin and allowed the purification of the complex alpha 2 beta 1, also termed Very Late Antigen 2 (VLA-2). It did not recognize human beta 1 integrin.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D010980 Platelet Membrane Glycoproteins Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors. PM-GP,Platelet Glycoprotein,Platelet Membrane Glycoprotein,PM-GPs,Platelet Glycoproteins,Glycoprotein, Platelet,Glycoprotein, Platelet Membrane,Glycoproteins, Platelet,Glycoproteins, Platelet Membrane,Membrane Glycoprotein, Platelet,Membrane Glycoproteins, Platelet,PM GP
D001792 Blood Platelets Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation. Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D006084 Graft Rejection An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient. Transplant Rejection,Rejection, Transplant,Transplantation Rejection,Graft Rejections,Rejection, Graft,Rejection, Transplantation,Rejections, Graft,Rejections, Transplant,Rejections, Transplantation,Transplant Rejections,Transplantation Rejections
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal

Related Publications

C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
January 1984, Human immunology,
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
February 1987, Molecular immunology,
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
June 1980, Journal of immunology (Baltimore, Md. : 1950),
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
January 2000, Archives of medical research,
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
November 1987, Journal of clinical microbiology,
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
January 1987, The International journal of biological markers,
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
March 1987, Proceedings of the National Academy of Sciences of the United States of America,
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
December 1992, Experimental and molecular pathology,
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
October 2015, Viral immunology,
C Richard, and K Thibaudeau, and B Charreau, and M J Loirat, and J Naulet, and D Blanchard, and J P Soulillou, and J F Bouhours
December 1991, Hybridoma,
Copied contents to your clipboard!