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Epithelial cell cultures became an experimental model employed for both animal and human studies. We established a modified method of culture of human nasal epithelial cells from nasal polyps using serum-free, hormonally supplemented Ham's F-12 medium and Vitrogen 100 collagen matrix. Cells reached confluent monolayer structures in 5 to 7 days (mean time 6 +/- 0.89 days) of culture. The confluent cultures consisted of pure epithelial cells, which was confirmed by light and electron microscopy, and immunohistochemical staining with anticytokeratin antibody. The success ratio of cultures was 61.5%. The culture system described is efficient enough to provide pure epithelial cells for further functional studies.
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
December 1985,
The American review of respiratory disease,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
September 2011,
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
March 2014,
Acta oto-laryngologica,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
October 1982,
Cell biology international reports,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
September 1989,
Laboratory investigation; a journal of technical methods and pathology,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
October 1988,
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
June 2004,
The Annals of otology, rhinology, and laryngology,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
June 2003,
Zhonghua er bi yan hou ke za zhi,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
May 1985,
Infection and immunity,
M L Kowalski, and
R Pawliczak, and
J Woźniak, and
K Siuda, and
J Grzegorczyk, and
M Pietrzak, and
Z Kozłowski, and
K Marek
June 1989,
Cell biology international reports,
