BIOMAT Database Logo BIOMAT Database Logo

Cloning and characterization of a novel class II phosphoinositide 3-kinase containing C2 domain. 1998

H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Institute of Life Science, Kurume University, Japan.

Phosphoinositide 3-kinases (PI3Ks) have been shown to play critical roles in cell growth, differentiation, survival, and vesicular transport. Class II PI3Ks have been recently identified in mouse and human (PI3K-C2 alpha/m-p170/m-cpk and HsC2-PI3K) and in Drosophila (PI3K 68D/cpk) which contain C2 domain at the C-terminus. However, their physiological function is largely unknown. We report here cloning and characterization of murine PI3K-C2 gamma, a novel class II PI3K. The catalytic domain as well as C2 domain are highly conserved in the Class II PI3K family, while the N-terminal regions of these proteins share little similarity. Unlike other Class II PI3Ks, PI3K-C2 gamma exclusively expressed in the liver, and a N-terminal truncated form was found in lung and a certain hematopoietic cell line. Specific antiserum against PI3K-C2 gamma precipitated PI3K activity from the membrane fraction of mouse liver but not from heart. Recombinant PI3K-C2 gamma exhibited a restricted lipid substrate specificity; it phosphorylated phosphatidylinositol (PtdIns) and PtdIns4P but not PtdIns(4,5)P2. Deletion mutations revealed that both the N-terminal region and the C2 domain were critical for enzymatic activity. The murine PI3K-C2 gamma gene locus was mapped to the distal region of mouse chromosome 6 in a region of homology with human chromosome 12p, which is distinct from the position of HsC2-PI3K. Cloning and biochemical characterization of the third member of class II PI3Ks provide a new insight into the function of this subfamily of PI3Ks.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D014018 Tissue Distribution Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios. Distribution, Tissue,Distributions, Tissue,Tissue Distributions
D017124 Conserved Sequence A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences. Conserved Sequences,Sequence, Conserved,Sequences, Conserved

Related Publications

H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K.
April 1997, Biochemical and biophysical research communications,
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
cDNA cloning of a third human C2-domain-containing class II phosphoinositide 3-kinase, PI3K-C2gamma, and chromosomal assignment of this gene (PIK3C2G) to 12p12.
December 1998, Genomics,
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain.
June 1996, The Journal of biological chemistry,
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Mouse p170 is a novel phosphatidylinositol 3-kinase containing a C2 domain.
June 1996, The Journal of biological chemistry,
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Cloning, expression, purification, and characterization of the human Class Ia phosphoinositide 3-kinase isoforms.
June 2004, Protein expression and purification,
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Crystal structure of the C2 domain of class II phosphatidylinositide 3-kinase C2alpha.
February 2006, The Journal of biological chemistry,
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin.
August 1997, The Biochemical journal,
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Class II phosphoinositide 3-kinase defines a novel signaling pathway in cell migration.
June 2005, The Journal of cell biology,
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α.
December 2018, Structure (London, England : 1993),
H Misawa, and M Ohtsubo, and N G Copeland, and D J Gilbert, and N A Jenkins, and A Yoshimura
Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase.
August 1995, Science (New York, N.Y.),
Copied contents to your clipboard!