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It has been demonstrated that the epidermal growth factor (EGF) stimulates migration of A-431 cells into the wound created in a monolayer in the absence of any significant effect on the incorporation of labeled thymidine into cells. The effect of EGF on migration is stimulated by the simultaneous addition of insulin. Cell treatment with mitomycin C completely eliminates the stimulating effect of EGF, without, however, affecting baseline level of cell migration, which is independent on growth stimulants. These results lead to the conclusion that the effect of EGF on cell migration and cell proliferation in A-431 culture can be partially separated.
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