-transparent.png){kind=logo}
Purification and characterisation of an intracellular X-prolyl-dipeptidyl aminopeptidase from Streptococcus thermophilus ACA-DC 4. 1997
Laboratory of Dairy Research, Agricultural University of Athens, Greece. et@auadec.aua.ariadne-t.gr
An intracellular X-prolyl-dipeptidyl aminopeptidase from Streptococcus thermophilus ACA-DC 4, isolated from traditional Greek yoghurt, was purified by anion exchange and gel filtration chromatography. A single band of molecular weight of about 80,000 appeared in SDS-PAGE; by gel filtration it was shown that the native enzyme was dimeric. The peptidase showed optimum activity on glycyl-prolyl 4-nitroanilide at pH 7.0 and at 50 degrees C, with K(m) = 3.1 mM and Vmax = 3500 U mg-1; over 50 degrees C the enzyme activity declined rapidly. It was inactivated by PMSF; sulfhydryl group reagents and metal chelators had little effect on enzyme activity.
Related Publications
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
April 1987,
Journal of dairy science,
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
March 1992,
The Journal of applied bacteriology,
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
January 1992,
International journal of food microbiology,
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
May 2018,
Food chemistry,
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
October 1995,
Proteins,
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
December 1995,
Microbiology (Reading, England),
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
January 1980,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
April 1998,
Journal of applied microbiology,
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
August 1976,
Analytical biochemistry,
E Tsakalidou, and
R Anastasiou, and
K Papadimitriou, and
E Manolopoulou, and
G Kalantzopoulos
April 2001,
Applied and environmental microbiology,
