Enrichment medium for isolation of Campylobacter jejuni-Campylobacter coli. 1995

L A Sicinschi
Laboratory of Microbiology, N. Testemitsanu University of Medicine, Republica Moldova.

A kaseini-broth enrichment medium (KBEM) for the isolation improvement of Campylobacter jejuni-Campylobacter coli from stool samples is described. Isolation of Campylobacters from stool specimens by direct inoculation onto solid selective medium was compared with isolation after twenty-four hours enrichment at 37 degrees C in KBEM, followed by subculture onto the same solid selective medium. Of 156 examined stool samples from diarrhoeal children positive results were obtained from 17 patients altogether: 11 by direct inoculation on selective media and 6 only owing to enrichment. Thus, an increase of 35.3% in the isolation rate was obtained by using the enrichment medium. The same medium allows the preservation of isolates of Campylobacter jejuni-Campylobacter coli for 2 months and longer at 37 degrees C.

UI MeSH Term Description Entries
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D016123 Campylobacter jejuni A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals. Campylobacter fetus subsp. jejuni,Vibrio hepaticus,Vibrio jejuni
D017000 Campylobacter coli A species of gram-negative, rod-shaped bacteria isolated from the intestinal tract of swine, poultry, and man. It may be pathogenic. Vibrio coli

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