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A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was categorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes a xylanase having the molecular weight of 105 kilodaltons. A number of smaller truncated proteins with activities towards 4-methylumbelliferyl-beta-D-cellobioside and xylan were also produced. The enzyme hydrolyzed xylan to xylo-oligosaccharide, indicating typical activity of endo-beta-1,4-xylanase. This endoxylanase hydrolyzed carboxymethylcellulose without notable reduction of the viscosity as an exo-beta-1,4-glucanase, even though the enzyme exhibited very low levels of activity against other soluble and insoluble cellulosic substrates.
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
November 1994,
Applied microbiology and biotechnology,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
March 1990,
Biochemical and biophysical research communications,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
October 2004,
Canadian journal of microbiology,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
September 1999,
Bioscience, biotechnology, and biochemistry,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
August 1990,
Genetika,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
June 1990,
Biochemical and biophysical research communications,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
January 1985,
Doklady Akademii nauk SSSR,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
March 2008,
Bioresource technology,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
April 1989,
Applied and environmental microbiology,
K H Jung, and
K M Lee, and
H Kim, and
K H Yoon, and
S H Park, and
M Y Pack
April 1988,
Applied and environmental microbiology,
