BIOMAT Database Logo BIOMAT Database Logo

Ultrastructural and freeze fracture cilia morphology of trachea epithelium in apparently healthy small ruminants. 1998

F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Department of Veterinary Pathology, Federico II University, Naples, Italy.

Specimens of tracheal mucosa were obtained from ten adult apparently healthy small ruminants (five goats and five sheep) both by a fibre optic endoscope and in a slaughterhouse. Ultrastructurally, a total of 50,000 cilia were examined. Pathological cilia were found in all examined subjects. The prevalence of compound cilia showed a range of 0.3% to 3%. Intracytoplasmic and swollen cilia ranged from 0.2% to 0.5%. The microtubular pattern was examined in 4,000 cross-sectioned cilia and an abnormal pattern was found in 5-7%. Microtubular defects involved both peripheral and central doublets, being peripheral abnormalities the prevailing ones. A central plug of electron dense material was observed in 2-3% of the examined basal bodies. Rare basal bodies characterized by an abnormal spatial configuration were also shown. Freeze fracture studies revealed a ciliary necklace composed of 4 to 5 rows of intramembrane particles.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008855 Microscopy, Electron, Scanning Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY. Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D009092 Mucous Membrane An EPITHELIUM with MUCUS-secreting cells, such as GOBLET CELLS. It forms the lining of many body cavities, such as the DIGESTIVE TRACT, the RESPIRATORY TRACT, and the reproductive tract. Mucosa, rich in blood and lymph vessels, comprises an inner epithelium, a middle layer (lamina propria) of loose CONNECTIVE TISSUE, and an outer layer (muscularis mucosae) of SMOOTH MUSCLE CELLS that separates the mucosa from submucosa. Lamina Propria,Mucosa,Mucosal Tissue,Muscularis Mucosae,Mucous Membranes,Membrane, Mucous,Membranes, Mucous,Mucosae, Muscularis,Mucosal Tissues,Propria, Lamina,Tissue, Mucosal,Tissues, Mucosal
D002923 Cilia Populations of thin, motile processes found covering the surface of ciliates (CILIOPHORA) or the free surface of the cells making up ciliated EPITHELIUM. Each cilium arises from a basic granule in the superficial layer of CYTOPLASM. The movement of cilia propels ciliates through the liquid in which they live. The movement of cilia on a ciliated epithelium serves to propel a surface layer of mucus or fluid. (King & Stansfield, A Dictionary of Genetics, 4th ed) Motile Cilia,Motile Cilium,Nodal Cilia,Nodal Cilium,Primary Cilia,Primary Cilium,Cilium,Cilia, Motile,Cilia, Nodal,Cilia, Primary,Cilium, Motile,Cilium, Nodal,Cilium, Primary
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005614 Freeze Fracturing Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica. Fracturing, Freeze,Fracturings, Freeze,Freeze Fracturings
D006041 Goats Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP. Capra,Capras,Goat
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D014132 Trachea The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi. Tracheas

Related Publications

F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Cilia on bovine mammary epithelium: ultrastructural observations.
March 1989, Cell and tissue research,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Pathological cilia in the tracheal mucosa of apparently healthy pigs.
October 1996, Journal of submicroscopic cytology and pathology,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Freeze-fracture morphology of biological membranes.
September 1978, Biochimica et biophysica acta,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Freeze-fracture morphology of nuclear pockets.
January 1991, Virchows Archiv. B, Cell pathology including molecular pathology,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Ultrastructural characterization of epithelial cell membranes in normal human conducting airway epithelium: A freeze-fracture study.
August 1985, The American journal of anatomy,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Geographical variations in the morphology of the small intestinal mucosa in apparently healthy individuals.
January 1973, Pathologia et microbiologia,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Atypical cilia in the oviductal epithelium of healthy cats.
April 1995, Journal of submicroscopic cytology and pathology,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Atypical cilia in the oviductal epithelium of healthy dogs.
October 1998, Journal of submicroscopic cytology and pathology,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Atypical cilia in the tracheal epithelium of healthy horses.
August 1991, Journal of comparative pathology,
F Roperto, and M Langella, and G Oliva, and B Restucci, and E Varricchio, and R Dallai
Freeze-fracture analysis of membrane events during early neogenesis of cilia in Tetrahymena: changes in fairy-ring morphology and membrane topography.
March 1983, Journal of cell science,
Copied contents to your clipboard!