Real time measurement of stimulated dopamine release in the conscious rat using fast cyclic voltammetry: dopamine release is not observed during intracranial self stimulation. 1998

Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
Department of Pharmacology, Queen Mary and Westfield College, London, UK. zyg.kruk@port.ac.uk

Fast cyclic voltammetry (FCV) was used to measure real time release of electrically stimulated endogenous dopamine in the nucleus accumbens (NAc) of conscious freely moving rats for up to 17 days. The method of electrode construction, implantation, electrical stimulation and recording of changes of extracellular dopamine concentration in the conscious rat are described. Rats trained on a continuous reinforcement schedule to perform intracranial self stimulation (ICSS) were implanted with electrodes for FCV. During ICSS, no faradaic signal was observed at an electrode implanted in the NAc. Decreasing the intensity of the stimulating current abolished ICSS, increasing the stimulating current disrupted ICSS. Operator delivered electrical stimulations using currents greater than those needed for ICSS yielded dopamine signals. It is concluded that during ICSS, sufficient dopamine does not reach the extracellular fluid space to yield a faradaic signal detectable by FCV.

UI MeSH Term Description Entries
D008297 Male Males
D008839 Microelectrodes Electrodes with an extremely small tip, used in a voltage clamp or other apparatus to stimulate or record bioelectric potentials of single cells intracellularly or extracellularly. (Dorland, 28th ed) Electrodes, Miniaturized,Electrode, Miniaturized,Microelectrode,Miniaturized Electrode,Miniaturized Electrodes
D004298 Dopamine One of the catecholamine NEUROTRANSMITTERS in the brain. It is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action. Hydroxytyramine,3,4-Dihydroxyphenethylamine,4-(2-Aminoethyl)-1,2-benzenediol,Dopamine Hydrochloride,Intropin,3,4 Dihydroxyphenethylamine,Hydrochloride, Dopamine
D004558 Electric Stimulation Use of electric potential or currents to elicit biological responses. Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D004563 Electrochemistry The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes. Electrochemistries
D004567 Electrodes, Implanted Surgically placed electric conductors through which ELECTRIC STIMULATION is delivered to or electrical activity is recorded from a specific point inside the body. Implantable Electrodes,Implantable Stimulation Electrodes,Implanted Electrodes,Implanted Stimulation Electrodes,Electrode, Implantable,Electrode, Implantable Stimulation,Electrode, Implanted,Electrode, Implanted Stimulation,Electrodes, Implantable,Electrodes, Implantable Stimulation,Electrodes, Implanted Stimulation,Implantable Electrode,Implantable Stimulation Electrode,Implanted Electrode,Implanted Stimulation Electrode,Stimulation Electrode, Implantable,Stimulation Electrode, Implanted,Stimulation Electrodes, Implantable,Stimulation Electrodes, Implanted
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001522 Behavior, Animal The observable response an animal makes to any situation. Autotomy Animal,Animal Behavior,Animal Behaviors
D012653 Self Stimulation The act or process of inducing or increasing the level of arousal in oneself. It can be observed in various situations; for example, infants who are understimulated may explore their surroundings or babble to themselves.(https://dictionary.apa.org/self-stimulation accessed 12/30/2020) ICSS Intracranial Self-Stimulation,Intracranial Self Stimulation,Self-Stimulation,Intracranial Self Stimulations,Self Stimulation, Intracranial,Self Stimulations,Self Stimulations, Intracranial,Self-Stimulations,Stimulation, Self,Stimulations, Self
D013238 Stereotaxic Techniques Techniques used mostly during brain surgery which use a system of three-dimensional coordinates to locate the site to be operated on. Stereotactic Techniques,Stereotaxic Technics,Stereotactic Technique,Stereotaxic Technic,Stereotaxic Technique,Technic, Stereotaxic,Technics, Stereotaxic,Technique, Stereotactic,Technique, Stereotaxic,Techniques, Stereotactic,Techniques, Stereotaxic

Related Publications

Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
November 2016, ACS chemical neuroscience,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
April 2018, Journal of neuroscience methods,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
September 1990, Journal of neuroscience methods,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
November 2023, ACS sensors,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
July 2018, Analytical chemistry,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
January 2009, Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
October 2018, Bio-protocol,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
February 1990, Brain research,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
March 1996, Journal of neurochemistry,
Z L Kruk, and S Cheeta, and J Milla, and R Muscat, and J E Williams, and P Willner
August 1986, Brain research,
Copied contents to your clipboard!