Trapping of DNA in nonuniform oscillating electric fields. 1998

C L Asbury, and G van den Engh
Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.

DNA molecules can be manipulated in aqueous solution in a manner analogous to optical trapping. Due to the induction of an electric dipole, DNA molecules are pulled by a gradient force to regions of high electric field strength. Molecules can be locally trapped in an oscillating field using strips of very thin gold film to generate strong electric fields with steep gradients. Spatial control over the trapped molecules is achieved because they are confined to a width of approximately 5 microm along the edges of the gold-film strips. By mixing static and oscillating electric fields, trapped molecules can be moved from one edge to another or made to follow precise trajectories along the edges. This phenomenon should be useful in microdevices for manipulation of small quantities or single molecules of DNA.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D009991 Oscillometry The measurement of frequency or oscillation changes. Oscillometries
D010582 Bacteriophage lambda A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection. Coliphage lambda,Enterobacteria phage lambda,Phage lambda,lambda Phage
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D004558 Electric Stimulation Use of electric potential or currents to elicit biological responses. Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D004563 Electrochemistry The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes. Electrochemistries
D012996 Solutions The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed) Solution
D018715 Microscopy, Video Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY. Video Microscopy,Videomicrography,Videomicroscopy,Microscopies, Video,Video Microscopies,Videomicrographies,Videomicroscopies

Related Publications

C L Asbury, and G van den Engh
February 1978, Science (New York, N.Y.),
C L Asbury, and G van den Engh
January 2011, Chemical communications (Cambridge, England),
C L Asbury, and G van den Engh
October 1991, Physical review. B, Condensed matter,
C L Asbury, and G van den Engh
June 2003, Analytical and bioanalytical chemistry,
C L Asbury, and G van den Engh
January 2012, Annual review of physical chemistry,
C L Asbury, and G van den Engh
January 1981, Neuroscience and behavioral physiology,
C L Asbury, and G van den Engh
May 1979, Fiziologicheskii zhurnal SSSR imeni I. M. Sechenova,
C L Asbury, and G van den Engh
February 1999, Biophysical journal,
C L Asbury, and G van den Engh
February 2018, Langmuir : the ACS journal of surfaces and colloids,
C L Asbury, and G van den Engh
April 2008, Langmuir : the ACS journal of surfaces and colloids,
Copied contents to your clipboard!