Biomaterial Database

High-pressure-induced hemolysis of hereditary spherocytic erythrocytes is not suppressed by DIDS labeling. 1997

T Yamaguchi, and S Terada, and H Ideguchi

Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka, 814-80 Japan. takeo@ssat.fukuoka-u.ac.jp

Hemolytic properties of human erythrocytes in hereditary spherocytosis (HS) were examined under hydrostatic pressure or hypotonic conditions. In the hypotonic buffer, HS erythrocytes were more fragile than normal erythrocytes, and the osmotic fragility was similarly enhanced if both erythrocytes were treated with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), an anion transport inhibitor. On the other hand, the hemolysis of HS erythrocytes at 200 MPa was almost the same degree as that of normal cells. Upon DIDS treatment, the hemolysis of normal erythrocytes at 200 MPa was suppressed by about 35%, whereas that of HS erythrocytes was not affected. The absence of a suppressive effect of DIDS on high-pressure-induced hemolysis is likely to be HS-specific and may be a reflection of the underlying defects of band 3-cytoskeleton interactions in HS erythrocytes.

UI	MeSH Term	Description	Entries
D007038	Hypotonic Solutions	Solutions that have a lesser osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.	Solutions, Hypotonic
D009996	Osmotic Fragility	RED BLOOD CELL sensitivity to change in OSMOTIC PRESSURE. When exposed to a hypotonic concentration of sodium in a solution, red cells take in more water, swell until the capacity of the cell membrane is exceeded, and burst.	Saline Fragility,Fragility, Osmotic,Fragility, Saline
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006461	Hemolysis	The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.	Haemolysis,Extravascular Hemolysis,Intravascular Hemolysis,Extravascular Hemolyses,Haemolyses,Hemolyses, Extravascular,Hemolyses, Intravascular,Hemolysis, Extravascular,Hemolysis, Intravascular,Intravascular Hemolyses
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006874	Hydrostatic Pressure	The pressure due to the weight of fluid.	Hydrostatic Pressures,Pressure, Hydrostatic,Pressures, Hydrostatic
D013103	Spherocytosis, Hereditary	A group of familial congenital hemolytic anemias characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. The erythrocytes have increased osmotic fragility and are abnormally permeable to sodium ions.	Hereditary Spherocytoses,Spherocytoses, Hereditary
D017136	Ion Transport	The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.	Antiport,Ion Cotransport,Ion Exchange, Intracellular,Symport,Uniport,Active Ion Transport,Facilitated Ion Transport,Passive Ion Transport,Cotransport, Ion,Exchange, Intracellular Ion,Intracellular Ion Exchange,Ion Transport, Active,Ion Transport, Facilitated,Ion Transport, Passive,Transport, Active Ion,Transport, Ion
D017878	4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid	An inhibitor of anion conductance including band 3-mediated anion transport.	4,4'-Diisothiocyano-2,2'-Stilbene Disulfonic Acid,DIDS