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Association of transcription factors with the nuclear matrix represents a mechanism by which nuclear architecture may influence transcriptional control of gene expression. This investigation examines nuclear matrix associated proteins (NMP's) isolated from ocular lens epithelial cells by monitoring DNA binding activities using consensus oligonucleotides recognized by the transcription factors YY1, AML-1, AP-1, SP-1 and ATF. The nuclear matrix fractions tested included an immortilized human lens epithelial cell line containing the SV40 large T-antigen, and two mouse lens epithelial cell lines derived from either a normal mouse or a cataract mouse. A rabbit epidermal epithelial cell line and HeLa cells were also included in this study for comparison. The data from these experiments reveal that ubiquitously represented and tissue restricted regulatory proteins are associated with nuclear matrix of lens epithelial cells. The functional significance of the nuclear matrix association of these transcription factors remains to be determined. However, our findings raise the possibility that the transcription factors associated with the nuclear matrix could have specific roles in gene regulation and eye tissue development.
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
May 1995,
Journal of cellular biochemistry,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
December 1978,
Journal of cell science,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
June 1991,
The Journal of biological chemistry,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
March 2007,
Journal of cellular biochemistry,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
May 1999,
The Plant journal : for cell and molecular biology,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
January 1997,
Methods in cell biology,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
August 2001,
Journal of cataract and refractive surgery,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
May 1985,
Biochimica et biophysica acta,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
March 1993,
FEBS letters,
M Bagchi, and
S A Ansari, and
D M Lindenmuth, and
A J van Wijnen, and
J Lian, and
J L Stein, and
G S Stein
October 1992,
Nucleic acids research,
